Kemp P, Lander D J, Orpin C G
J Gen Microbiol. 1984 Jan;130(1):27-37. doi: 10.1099/00221287-130-1-27.
The major phospholipids of the anaerobic rumen phycomycete Piromonas communis were phosphatidylethanolamine (38%), phosphatidylcholine (26%) and phosphatidylinositol (13%); no sphingolipids, glycolipids, plasmalogens or phosphonyl lipids were detected. Free fatty acids, triacylglycerols, 1:2 diacylglycerols and a variable amount of 1:3 diacylglycerol were identified, as were minor amounts of squalene and a triterpenol which is probably tetrahymanol. Approximately half the fatty acids were straight chain, even 12 to 24 carbon, saturated acids, the remainder being even 16 to 24 carbon, mono-unsaturated fatty acids. The double bonds in all except the 16 carbon acid were in the omega 9 position. The unsaturation is introduced by a delta 9 desaturase which uses stearic acid as substrate and which does not use oxygen as a terminal electron acceptor. 14C from acetate and glucose was incorporated into the fatty acids of all complex lipids, as were lauric, myristic, palmitic, stearic and oleic acids. [14C]Choline was incorporated into phosphatidylcholine and [14C]ethanolamine into phosphatidylethanolamine and phosphatidylcholine. Label from [14C]serine was recovered in phosphatidylserine and phosphatidylethanolamine, but was not detected in phosphatidylcholine.
厌氧瘤胃藻状菌普通梨形菌的主要磷脂为磷脂酰乙醇胺（38%）、磷脂酰胆碱（26%）和磷脂酰肌醇（13%）；未检测到鞘脂、糖脂、缩醛磷脂或膦酰脂质。鉴定出了游离脂肪酸、三酰甘油、1:2二酰甘油和可变数量的1:3二酰甘油，还有少量的角鲨烯和一种可能是四膜虫醇的三萜醇。大约一半的脂肪酸是直链的，为12至24个碳的偶数碳饱和酸，其余为16至24个碳的偶数碳单不饱和脂肪酸。除16碳脂肪酸外，所有脂肪酸的双键都在ω9位置。这种不饱和度是由一种以硬脂酸为底物且不以氧作为末端电子受体的Δ9去饱和酶引入的。来自乙酸盐和葡萄糖的14C被掺入到所有复合脂质的脂肪酸中，月桂酸、肉豆蔻酸、棕榈酸、硬脂酸和油酸也是如此。[14C]胆碱被掺入到磷脂酰胆碱中，[14C]乙醇胺被掺入到磷脂酰乙醇胺和磷脂酰胆碱中。[14C]丝氨酸的标记在磷脂酰丝氨酸和磷脂酰乙醇胺中被回收，但在磷脂酰胆碱中未检测到。
