Matuoka S, Shichida Y, Yoshizawa T
Biochim Biophys Acta. 1984 Apr 26;765(1):38-42. doi: 10.1016/0005-2728(84)90154-3.
Excitation of squid rhodopsin with a single laser pulse (532 nm, 25 ps) at 18 degrees C yielded photorhodopsin, a precursor of bathorhodopsin. In the linear region, no relation between amount of photorhodopsin and excitation-energy hypsorhodopsin was detected, while in a photon saturation region this was observed. The time constant of hypsorhodopsin to bathorhodopsin decay was about 125 ps. Dependencies of formation of photorhodopsin and hypsorhodopsin on the excitation energy suggest that hypsorhodopsins of squid and octopus are formed by a two-photon reaction. No cattle hypsorhodopsin was detected in our experimental conditions.
在18摄氏度下,用单个激光脉冲(532纳米,25皮秒)激发鱿鱼视紫红质产生了光视紫红质,它是视紫红质原的前体。在线性区域,未检测到光视紫红质的量与激发能视紫红质之间的关系,而在光子饱和区域观察到了这种关系。视紫红质原向视紫红质衰变的时间常数约为125皮秒。光视紫红质和视紫红质原的形成对激发能的依赖性表明,鱿鱼和章鱼的视紫红质原是由双光子反应形成的。在我们的实验条件下未检测到牛视紫红质原。