Turc-Carel C, Philip I, Berger M P, Philip T, Lenoir G M
Cancer Genet Cytogenet. 1984 May;12(1):1-19. doi: 10.1016/0165-4608(84)90002-5.
A detailed banded chromosome analysis was performed in five established Ewing's sarcoma (ES) cell lines originating from four unrelated patients in relapse. Of various numerical and structural abnormalities, a reciprocal translocation between chromosomes #11 and #22, t(11;22)(q24;q12), was observed in four of the lines. The t(11;22) was seen in every cell in three lines; in the fourth, it was seen in only 21% of the cells considered stemline, but the der(22) was present in the remaining 79% of cells. These results suggest that t(11;22)(q24;q12) is a chromosomal change specific to ES cells, in which the rearrangement of chromosome #22 could be the consistent karyotypic feature and the crucial step in terms of cell proliferation. Other, nonrandom chromosomal changes were found: monosomies 2p11----2pter, 10q25----10qter, and 17pter----17q11, and partial trisomies 1q21----1q31 and 8q24.1----8q24.2. The role of the therapeutic regimen received by these patients must be evaluated with regard to the formation of a wide variety of homogeneously staining regions, which were observed in every cell line, particularly on the short arm of chromosome #7, which was observed in three of the five cell lines.
对源自4名复发的不相关患者的5个已建立的尤因肉瘤（ES）细胞系进行了详细的染色体显带分析。在各种数目和结构异常中，在4个细胞系中观察到11号和22号染色体之间的相互易位，即t(11;22)(q24;q12)。在3个细胞系的每个细胞中都可见到t(11;22)；在第4个细胞系中，仅在21%被认为是主干系的细胞中可见到，但在其余79%的细胞中存在der(22)。这些结果表明，t(11;22)(q24;q12)是ES细胞特有的染色体变化，其中22号染色体的重排可能是一致的核型特征，也是细胞增殖方面的关键步骤。还发现了其他非随机染色体变化：2号染色体短臂（2p11----2pter）、10号染色体长臂（10q25----10qter）和17号染色体（17pter----17q11）单体，以及1号染色体长臂（1q21----1q31）和8号染色体（8q24.1----8q24.2）部分三体。必须评估这些患者接受的治疗方案对形成各种均匀染色区的作用，在每个细胞系中都观察到了均匀染色区，特别是在5个细胞系中的3个中观察到的7号染色体短臂上。
