Bergqvist Y, Eckerbom S
J Chromatogr. 1984 Mar 9;306:147-53. doi: 10.1016/s0378-4347(00)80877-4.
A gas chromatographic method for the simultaneous determination of therapeutic levels of chloroquine, and the metabolites deethyl chloroquine and bideethyl chloroquine in human whole blood, plasma and urine has been developed by use of the capillary column technique. The analytes were extracted as bases with n-hexane-1-pentanol (90:10) and re-extracted into an acidic aqueous phase. After a further extraction to a small volume of chloroform, the substances were injected by the falling needle technique onto a fused-silica capillary column followed by nitrogen-selective detection. Limits of determination using 2 ml of the sample were found to be 10 nmol/l (3 ng/ml) for chloroquine and deethyl chloroquine and 30-40 nmol/l (10 ng/ml) for bideethyl chloroquine with a coefficient of variation of less than 15%. The precision of the method at the 100 nmol/l level was about 4% (n = 8) for chloroquine.
已开发出一种气相色谱法，利用毛细管柱技术同时测定人全血、血浆和尿液中氯喹及其代谢物去乙基氯喹和双去乙基氯喹的治疗浓度。分析物用正己烷 - 1 - 戊醇（90:10）作为碱进行萃取，然后再萃取到酸性水相中。进一步萃取至少量氯仿后，通过落针技术将这些物质注入熔融石英毛细管柱，随后进行氮选择性检测。使用2 ml样品时，氯喹和去乙基氯喹的测定限为10 nmol/l（3 ng/ml），双去乙基氯喹为30 - 40 nmol/l（10 ng/ml），变异系数小于15%。该方法在100 nmol/l水平下，氯喹的精密度约为4%（n = 8）。
