Mahfouz M M, Smith T L, Kummerow F A
Lipids. 1984 Mar;19(3):214-22. doi: 10.1007/BF02534800.
Four groups of rats were fed diets containing 15% (w/w) high-oleic safflower oil (SFO, rich in cis-18:1 acids), a mixture of 80% partially hydrogenated soybean oil plus 20% corn oil (H + CO, rich in trans-18:1 acids), lard (L, rich in saturated fatty acids) and corn oil (Co, rich in 18:2 omega 6). Fatty acid composition of liver microsomes and activities of the delta 5, delta 6 and delta 9 desaturases were determined. Microsomal delta 6 desaturase activity and arachidonic acid were lower in the H + CO group compared with SFO of L. No difference was found in the delta 5 or delta 6 desaturase activity of CO and SFO groups. Thus, the oleic-acid level of the SFO diet had no effect on the metabolism of 18:2 omega 6. Fluorescent polarization studies, using trans-parinaric acid as a probe, showed no differences between the physical states of phospholipid vesicles made from lipids isolated from each group. We concluded that the trans-18:1 acids in partially hydrogenated soybean oil have a more inhibitory effect than saturated acids on EFA metabolism, even in the presence of adequate amounts of essential fatty acid.
将四组大鼠分别喂食含有15%（重量/重量）高油酸红花油（SFO，富含顺式-18:1酸）、80%部分氢化大豆油加20%玉米油的混合物（H + CO，富含反式-18:1酸）、猪油（L，富含饱和脂肪酸）和玉米油（Co，富含18:2ω6）的日粮。测定了肝微粒体的脂肪酸组成以及δ5、δ6和δ9去饱和酶的活性。与SFO组或L组相比，H + CO组的微粒体δ6去饱和酶活性和花生四烯酸含量较低。CO组和SFO组的δ5或δ6去饱和酶活性未发现差异。因此，SFO日粮中的油酸水平对18:2ω6的代谢没有影响。使用反式-十八碳四烯酸作为探针的荧光偏振研究表明，从每组分离的脂质制成的磷脂囊泡的物理状态之间没有差异。我们得出结论，即使在存在足够量必需脂肪酸的情况下，部分氢化大豆油中的反式-18:1酸对必需脂肪酸代谢的抑制作用也比饱和酸更强。
