Daily B, Elson E L, Zahalak G I
Biophys J. 1984 Apr;45(4):671-82. doi: 10.1016/S0006-3495(84)84209-5.
Cell poking, a new method for measuring mechanical properties of single cells was used to determine the elastic area compressibility modulus of osmotically swollen human erythrocytes. With this method we determined the force required to indent cells attached to a glass coverslip (Petersen, N.O., W. B. McConnaughey , and E. L. Elson , 1982, Proc. Natl. Acad. Sci. USA, 79:5327. Forces on the order of one millidyne and indentations on the order of one micron were detected. An analysis of these data in terms of a simplified mechanical model yielded the elastic area compressibility modulus. This analysis used a variational approach to minimize the isothermal elastic potential energy density function given by E. A. Evans and R. Skalak (Mechanics and Thermodynamics of Biomembranes, 1980, CRC Press, Boca Raton , FL). Measurements on swollen erythrocytes gave a range of values, depending in part on the osmotic conditions, of 17.9 +/- 8.2 to 34.8 +/- 12.0 mdyn /micron for the elastic area compressibility modulus at 25 degrees C. Fractional area expansion greater than 2.6 +/- 0.8% produced rapid cell lysis. These values were not corrected for the reversible movement of water across the cell membrane in response to hydrostatic pressure gradients. Our results agree reasonably with those obtained by Evans et al. (Evans, E.A., R. Waugh , and L. Melnick , 1976, Biophys. J., 16:585-595.) using micropipette aspiration under similar conditions.
细胞针刺法是一种测量单细胞力学特性的新方法，用于测定渗透肿胀的人红细胞的弹性面积压缩模量。通过这种方法，我们确定了压入附着在玻璃盖玻片上的细胞所需的力（彼得森，N.O.，W.B.麦康纳吉，和E.L.埃尔森，1982年，《美国国家科学院院刊》，79:5327。检测到的力约为一毫达因，压痕约为一微米。根据一个简化的力学模型对这些数据进行分析，得出了弹性面积压缩模量。该分析采用变分法，以最小化由E.A.埃文斯和R.斯卡拉克给出的等温弹性势能密度函数（《生物膜的力学与热力学》，1980年，CRC出版社，佛罗里达州博卡拉顿）。对肿胀红细胞的测量给出了一系列值，部分取决于渗透条件，在25摄氏度下，弹性面积压缩模量为17.9±8.2至34.8±12.0毫达因/微米。大于2.6±0.8%的面积分数膨胀会导致细胞迅速裂解。这些值未针对由于静水压力梯度导致的水在细胞膜上的可逆移动进行校正。我们的结果与埃文斯等人（埃文斯，E.A.，R.沃，和L.梅尔尼克，1976年，《生物物理学杂志》，16:585 - 595）在类似条件下使用微吸管抽吸法得到的结果相当一致。
