Cook R M, Moran D M
Int Arch Allergy Appl Immunol. 1984;74(3):216-20. doi: 10.1159/000233546.
The ability of inflammatory peritoneal exudate cells to modify a murine lymphoproliferative response to rye grass pollen extract was investigated. Popliteal and mesenteric lymph node cells from Balb/c mice immunised with either aqueous rye or rye grass emulsified in complete Freund's adjuvant were co-cultured with 2 X 10(3)-10(5) macrophage or polymorphonuclear neutrophil (PMN)-enriched exudate cells. There was a dose-dependent increase in the response to rye grass antigen with increasing numbers of PMN. A similar enhancement was seen with up to 10(4) macrophages per culture; higher numbers were inhibitory. Lymph node cells from rye grass or ovalbumin-immune mice that had been challenged with a combination of sensitising antigen plus 10(7) PMN gave an increased antigen-specific response in vitro. Neither antigen alone, PMN nor a mixture of PMN plus heterologous antigen was active. Adoptive transfer of a similar number of peritoneal macrophages plus antigen was also without effect. These results are in accord with the suggestion that cells obtained from acute inflammatory lesions can directly influence the activity of immunocompetent lymphocytes.
研究了炎性腹膜渗出细胞改变小鼠对黑麦草花粉提取物的淋巴细胞增殖反应的能力。将用完全弗氏佐剂乳化的含水黑麦或黑麦草免疫的Balb/c小鼠的腘窝和肠系膜淋巴结细胞与2×10³ - 10⁵富含巨噬细胞或多形核中性粒细胞（PMN）的渗出细胞共培养。随着PMN数量的增加，对黑麦草抗原的反应呈剂量依赖性增加。每培养物中多达10⁴个巨噬细胞时也观察到类似的增强作用；更高数量则具有抑制作用。用致敏抗原加10⁷个PMN攻击的黑麦草或卵清蛋白免疫小鼠的淋巴结细胞在体外产生了增强的抗原特异性反应。单独的抗原、PMN或PMN加异源抗原的混合物均无活性。类似数量的腹膜巨噬细胞加抗原的过继转移也没有效果。这些结果与以下观点一致，即从急性炎症病变中获得的细胞可直接影响免疫活性淋巴细胞的活性。
