Akerlund H E
J Biochem Biophys Methods. 1984 May;9(2):133-41. doi: 10.1016/0165-022x(84)90004-6.
An apparatus is described that reduces the time required for counter-current distribution in aqueous polymer two-phase systems by a factor of more than five. Phase settling, which has been the time consuming step, is here facilitated by centrifugation. The crucial point in the construction, that allows automatization, is that the transfer step in the counter-current distribution cycle is performed during centrifugation.
本文描述了一种装置,该装置可将水相聚合物两相系统中逆流分配所需的时间缩短五倍以上。一直以来耗时的相沉降步骤在此通过离心得到了促进。该装置构造的关键点在于,其逆流分配循环中的转移步骤是在离心过程中进行的,这使得自动化成为可能。