Bonnett R, Buckley D G, Hamzetash D, Hawkes G E, Ioannou S, Stoll M S
Biochem J. 1984 May 1;219(3):1053-6. doi: 10.1042/bj2191053.
An improved preparation of photobilirubin II in ammoniacal methanol is described. Evidence is presented which distinguishes between the two structures proposed earlier for photobilirubin II in favour of the cycloheptadienyl structure. Nuclear-Overhauser-enhancement measurements with bilirubin IX alpha and photobilirubin II in dimethyl sulphoxide are complicated by the occurrence of negative and zero effects. The partition coefficient of photobilirubin II between chloroform and phosphate buffer (pH 7.4) is 0.67.
本文描述了一种在氨性甲醇中制备光胆红素II的改进方法。文中给出的证据区分了先前提出的光胆红素II的两种结构，支持环庚二烯基结构。在二甲亚砜中对胆红素IXα和光胆红素II进行核Overhauser增强测量时，由于出现负效应和零效应而变得复杂。光胆红素II在氯仿和磷酸盐缓冲液（pH 7.4）之间的分配系数为0.67。
