Onishi S, Itoh S, Isobe K, Togari H, Kitoh H, Nishimura Y
Pediatrics. 1982 Mar;69(3):273-6.
Comparisons of serum concentrations of unknown pigment and photobilirubin IX alpha , the two main bilirubin photoproducts, were made during phototherapy in infants with and without bronze baby syndrome who were treated similarly. The serum concentrations of unknown pigment estimated by high-pressure liquid chromatography in infants with the bronze baby syndrome were significantly increased in comparison with those in the control hyperbilirubinemic neonates during phototherapy. However, there was no difference in the serum concentrations of photobilirubin IX alpha between infants with bronze baby syndrome and the control groups. The unknown pigment separated from bilirubin photoproducts obtained from experiments in vitro by high-pressure liquid chromatography was gradually decomposed into brown products that showed the absorption spectrum similar to that of the serum of infants with bronze baby syndrome. This fact is probably due to reduction in hepatic excretory function of bilirubin photoproducts, especially in known pigment, because its main excretory pathway is the biliary route. The pigment accumulated in the body may be polymerized and forms bilifuscin-like substances following a free radical reaction. It is concluded that the brown pigment is formed via unknown pigment.
在接受类似光疗的患有和未患青铜婴儿综合征的婴儿中，对两种主要胆红素光产物——未知色素和光胆红素IXα的血清浓度进行了比较。通过高压液相色谱法估计，患有青铜婴儿综合征的婴儿在光疗期间，其未知色素的血清浓度相较于对照高胆红素血症新生儿显著升高。然而，患有青铜婴儿综合征的婴儿与对照组之间，光胆红素IXα的血清浓度并无差异。通过高压液相色谱法从体外实验获得的胆红素光产物中分离出的未知色素，会逐渐分解为棕色产物，该棕色产物显示出与患有青铜婴儿综合征婴儿血清相似的吸收光谱。这一事实可能是由于胆红素光产物，尤其是已知色素的肝脏排泄功能降低所致，因为其主要排泄途径是胆汁途径。体内积累的色素可能会发生聚合，并在自由基反应后形成胆褐素样物质。得出的结论是，棕色色素是通过未知色素形成的。
