透明质酸盐在质膜上合成。
Hyaluronate is synthesized at plasma membranes.
作者信息
Prehm P
出版信息
Biochem J. 1984 Jun 1;220(2):597-600. doi: 10.1042/bj2200597.
Abstract
The hybrid cell B6 line, which synthesizes large amounts of hyaluronate as the predominant glycosaminoglycan, was grown in the presence of [3H]glucosamine. The [3H]hyaluronate has a high molecular weight and was excluded by Sephacryl S-1000. After disruption of the cells the [3H]hyaluronate could further be elongated by incubation with UDP-GlcNAc and UDP-[14C]GlcA, yielding a hybrid molecule of hyaluronate labelled with [3H]GlcNAc and [14C]GlcA. Treatment of the cells with hyaluronidase before disruption eliminated the large [3H]hyaluronate and elongation of nascent chains in vitro commenced from low-molecular-weight chains. Thus nascent hyaluronate chains were degraded extracellularly by hyaluronidase and were therefore synthesized at the inner side of plasma membranes and extruded to the cell surface.
摘要
杂交细胞B6系能合成大量透明质酸作为主要的糖胺聚糖，它在[3H]葡糖胺存在的情况下生长。[3H]透明质酸具有高分子量，不能通过Sephacryl S - 1000凝胶柱。细胞破碎后，[3H]透明质酸可通过与UDP - GlcNAc和UDP - [14C]GlcA一起温育进一步延长，产生一个用[3H]GlcNAc和[14C]GlcA标记的透明质酸杂交分子。在细胞破碎前用透明质酸酶处理细胞，消除了大分子的[3H]透明质酸，体外新生链的延长从低分子量链开始。因此，新生的透明质酸链在细胞外被透明质酸酶降解，所以它是在质膜内侧合成并被挤出到细胞表面的。
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