Warrington R J, Sauder P J, Wilkins J A, Rutherford W J
Immunol Lett. 1982 Jun;4(6):339-44. doi: 10.1016/0165-2478(82)90063-3.
Human plaque-forming cells (PFC) have been quantitated following pokeweed mitogen stimulation by a protein A plaque technique using human erythrocytes. The PFC are generated in cultures supplemented with non-mitogenic human AB serum of foetal bovine serum (FBS) of varying degrees of inherent mitogenicity. In the latter case, no correlation exists between the mitogenicity of the serum supplement used in these cultures and the PFC response obtained. This fact, in addition to the demonstrated usefulness of the AB sera in facilitating the generation of PFC makes it unlikely that serum mitogenicity is a requirement for in vitro human immunoglobulin synthesis and secretion.
通过使用人红细胞的蛋白A空斑技术，在商陆丝裂原刺激后对人空斑形成细胞（PFC）进行了定量。PFC是在补充有不同程度固有促有丝分裂活性的非促有丝分裂人AB血清或胎牛血清（FBS）的培养物中产生的。在后一种情况下，这些培养物中使用的血清补充剂的促有丝分裂活性与获得的PFC反应之间不存在相关性。这一事实，再加上已证明AB血清在促进PFC产生方面的有用性，使得血清促有丝分裂活性不太可能是体外人免疫球蛋白合成和分泌的必要条件。
