Joshi A, Verma M, Chakravorty M
Antimicrob Agents Chemother. 1982 Oct;22(4):541-7. doi: 10.1128/AAC.22.4.541.
Spontaneous mutants of Salmonella typhimurium isolated in our laboratory from thiolutin-containing tryptone agar plates are partially resistant to thiolutin in enriched media. In minimal media, they are not resistant. The mutants are not temperature sensitive but fail to support the development of phage P22 at higher temperatures (40 degrees C). Thiolutin did not interfere with RNA polymerase or nucleotide kinase in in vitro experiments. However, thiolutin did inhibit the rate of incorporation of exogenous uridine into the cellular pool and consequently the acid-precipitable material. It appears that one site of action of thiolutin is at the membrane level.
我们实验室从含硫藤黄素的胰蛋白胨琼脂平板上分离出的鼠伤寒沙门氏菌自发突变体，在富集培养基中对硫藤黄素具有部分抗性。在基本培养基中，它们没有抗性。这些突变体对温度不敏感，但在较高温度（40摄氏度）下无法支持噬菌体P22的生长。在体外实验中，硫藤黄素不干扰RNA聚合酶或核苷酸激酶。然而，硫藤黄素确实抑制了外源尿苷掺入细胞池的速率，从而抑制了酸沉淀物质的形成。看来硫藤黄素的一个作用位点是在膜水平。
