Sivasubramanian N, Jayaraman R
Mol Gen Genet. 1980;180(3):609-15. doi: 10.1007/BF00268068.
Two mutations in Escherichia coli conferring resistance to the transcription initiation inhibitor, thiolutin, have been mapped. One of these mutations (tln-I)( maps at 10.2 min on the genetic map and is cotransducible with dnaZ at a frequency of approximately 50%. The other mutation (tln-II) maps between metE and ilvD, probably close to rho, and is cotransducible with ilvD at a frequency of approximately 65%. The presence of both the mutations in the same cell confers resistance to thiolutin in minimal medium. Either one of them alone renders the cell 'conditionally auxotrophic' in the presence of the drug. The implications of these findings are discussed in relation to the mode of action of the thiolutin sensitive factors in transcription.
已对大肠杆菌中赋予对转录起始抑制剂硫藤黄素抗性的两种突变进行了定位。其中一种突变（tln-I）在遗传图谱上位于10.2分钟处，与dnaZ的共转导频率约为50%。另一种突变（tln-II）位于metE和ilvD之间，可能靠近rho，与ilvD的共转导频率约为65%。同一细胞中同时存在这两种突变可使细胞在基本培养基中对硫藤黄素产生抗性。单独存在其中任何一种突变都会使细胞在药物存在下“条件性营养缺陷”。结合硫藤黄素敏感因子在转录中的作用方式对这些发现的意义进行了讨论。
