Venables P J, Erhardt C C, Maini R N
Clin Exp Immunol. 1980 Jan;39(1):146-53.
Antibodies to extractable nuclear antigens (ENA) were analysed in the sera of fifty-two patients with severe rheumatoid arthritis (RA) who were divided into two categories: twenty-five with arthritis only and twenty-seven with extra-articular disease. Using haemagglutination, counterimmunoelectropheresis (CIE) and double diffusion, antibodies to ENA were detected in three (12%) of the patients with arthritis only. In the group with extra-articular disease, antibodies were found in sixteen (59%) of the patients. In ten patients the antibodies reacted with an RNase and DNAse resistant, but trypsin sensitive protein component of ENA. These patients all had extraarticular disease with digital vasculitis being a particularly common feature. Their sera also contained circulating immune complexes detected by elevated cryoprecipitate protein levels associated with relatively low complement levels. It is suggested that antibodies to soluble proteins of nuclear origin may be markers of circulating immune complexes in extra-articular RA.
对52例重症类风湿关节炎（RA）患者的血清进行了可提取核抗原（ENA）抗体分析，这些患者分为两类：25例仅有关节炎，27例有关节外疾病。采用血凝试验、对流免疫电泳（CIE）和双向扩散法，仅有关节炎的患者中3例（12%）检测到ENA抗体。在有关节外疾病的组中，16例（59%）患者检测到抗体。10例患者的抗体与ENA的一种对核糖核酸酶和脱氧核糖核酸酶有抗性但对胰蛋白酶敏感的蛋白质成分发生反应。这些患者均有关节外疾病，指端血管炎是一个特别常见的特征。他们的血清中还含有循环免疫复合物，通过冷沉淀蛋白水平升高且补体水平相对较低检测到。提示核源性可溶性蛋白抗体可能是关节外RA中循环免疫复合物的标志物。
