Kabelík V, Timoracký S, Cervenka P
Vet Med (Praha). 1980 Jun;25(6):359-66.
A description is presented of an adapted ultrathermostat, respecting the requirement for a continuous decrease in temperature by 1 degree C per minute, as necessary for keeping the frozen cultures alive. Viability was evaluated in the primary cells of calf kidneys in the first subpassage and permanent cell lines PK and TL 72 after their freezing and preservation at -192 degrees C for 30 days. In comparison with regularly passaged cultures, the frozen cells had a high viability.
本文描述了一种经过改良的超恒温器，它符合每分钟温度持续下降1摄氏度的要求，这是使冷冻培养物存活所必需的。在第一次传代时对小牛肾原代细胞以及永久细胞系PK和TL 72在-192摄氏度下冷冻保存30天后的活力进行了评估。与常规传代培养物相比，冷冻细胞具有较高的活力。
