McCarty K S, Wortman J, Stowers S, Lubahn D B, McCarty K S, Seigler H F
Cancer. 1980 Sep 15;46(6):1463-70. doi: 10.1002/1097-0142(19800915)46:6<1463::aid-cncr2820460628>3.0.co;2-q.
Melanomas from 20 patients were evaluated for estrogen and progesterone receptors. No restriction as to patient's age, sex, race, or menstrual status was made. Fifteen of the tumors were melanin producing. Of the 20 tumors examined, seven contained more than 3 fm/mg protein of specifically inhibitable estrogen binding. None of the tumors reported here demonstrated either a 4S or 8S binding protein as shown by sucrose density gradient analysis. All tumors in which estradiol binding was observed were melanin producing, whereas none of the amelanotic melanomas (five tumors) bound this steroid. Purified tyrosinase appeared to mimic the estrogen binding detected in melanoma cytosols, as demonstrated with the dextran-coated charcoal technique. Although the binding of estradiol to tyrosinase was inhibited by DOPA, the binding of estradiol to estrogen receptor preparations was not. These studies represent an extensive of previous studies of sex steroid binding in melanomas and suggest that gradient analysis and DOPA inhibition studies should be included in the evaluation of the estrogen binding phenomenon in human melanoma.
对20例患者的黑色素瘤进行了雌激素和孕激素受体评估。对患者的年龄、性别、种族或月经状态未作限制。其中15个肿瘤产生黑色素。在检测的20个肿瘤中，7个含有超过3 fm/mg蛋白质的特异性可抑制雌激素结合。此处报告的所有肿瘤经蔗糖密度梯度分析均未显示4S或8S结合蛋白。观察到雌二醇结合的所有肿瘤均产生黑色素，而无色素性黑色素瘤（5个肿瘤）均未结合该类固醇。如用葡聚糖包被活性炭技术所示，纯化的酪氨酸酶似乎模拟了黑色素瘤胞质溶胶中检测到的雌激素结合。虽然多巴可抑制雌二醇与酪氨酸酶的结合，但对雌二醇与雌激素受体制剂的结合无抑制作用。这些研究是对先前黑色素瘤中性类固醇结合研究的扩展，表明梯度分析和多巴抑制研究应纳入人类黑色素瘤雌激素结合现象的评估中。
