Mára M, Průchová J, John C, Smerák P, Sír Z
Folia Microbiol (Praha). 1981;26(1):52-8. doi: 10.1007/BF02927223.
The avirulent strain Listeria monocytogenes (Welshimer) induced upon a single injection 4 weeks prior to challenge with Mycobacterium tuberculosis H37Rv resistance in guinea-pigs, which was manifested by a significant decrease of spleen weight and Feldman's index in immunized animals. The degree of resistance was dependent on the immunizing dose and time of administration. Repeated high doses of Listeria yielded only a low or no effect. Further increase of resistance was obtained using the BCG lipids; however, stimulation of resistance with listeria lipids was not successful and, finally, Freund's incomplete adjuvant was significantly effective in the Feldman index only. The BCG lipids, extracted with ethanol, which contained nitrogen and water-soluble substances, induced a significant resistance against M. tuberculosis infection. The chloroform-methanol BCG lipids were also effective, however, significantly less than the ethanol-extracted material. The listeria factor Ei itself or together with Freund's incomplete adjuvant possessed only low effectiveness against mycobacterial infection. However, if injected together with ethanol-extracted BCG lipids, it produced a significant degree of resistance which was higher than that induced by lipids only. The degree of resistance was comparable with the effect of living BCG strain which served as a source of isolated lipids.
无毒力的单核细胞增生李斯特菌（韦尔希默氏菌）在豚鼠用结核分枝杆菌H37Rv攻击前4周单次注射后，诱导出了抵抗力，这表现为免疫动物的脾脏重量和费尔德曼指数显著降低。抵抗力的程度取决于免疫剂量和给药时间。重复高剂量的李斯特菌仅产生低效果或无效果。使用卡介苗脂质可进一步增强抵抗力；然而，用李斯特菌脂质刺激抵抗力未成功，最后，弗氏不完全佐剂仅在费尔德曼指数方面有显著效果。用乙醇提取的卡介苗脂质含有氮和水溶性物质，可诱导对结核分枝杆菌感染的显著抵抗力。氯仿 - 甲醇提取的卡介苗脂质也有效，但明显低于乙醇提取物。李斯特菌因子Ei本身或与弗氏不完全佐剂一起对分枝杆菌感染仅有低效力。然而，如果与乙醇提取的卡介苗脂质一起注射，它会产生显著程度的抵抗力，高于仅由脂质诱导的抵抗力。抵抗力的程度与用作分离脂质来源的活卡介苗菌株的效果相当。
