Bratescu A, Mayer E P, Teodorescu M
Infect Immun. 1981 Feb;31(2):816-21. doi: 10.1128/iai.31.2.816-821.1981.
In previous studies, we have shown that various lymphocyte subpopulations bind different strains of bacteria of different genera and species. Among these bacteria was a strain of Brucella melitensis which bound to all human B lymphocytes. To determine whether the binding of B. melitensis to human B lymphocytes was strain, species, or genus characteristic, we tested the binding of B. melitensis, Brucella abortus, Brucella ovis, Brucella suis, Brucella canis and Brucella neotomae to human normal and leukemic B lymphocytes. The binding of different Brucella species to B lymphocytes was determined by single- and double-labeling experiments in which a strain of Escherichia coli, coated with anti-light chain antibodies, was used as a marker for B cells. As in previous experiments, we found that B. melitensis and antibody-coated E. coli bound to the same cells. Also, we found that all the other species of bacteria tested bound to the B lymphocytes, normal or leukemic. B. ovis and B. neotomae, which are not human pathogens, bound to fewer B lymphocytes than did the human pathogens B. abortus, B. melitensis, B. suis, and B. canis. Furthermore, we found that the quality of rosettes formed by the nonpathogenic bacteria with the lymphocytes, i.e., the number of bacteria per lymphocytes, was lower than that of pathogenic Brucella species. We conclude that all of the Brucella species tested have the ability to bind to human B lymphocytes, but that only those which are human pathogens bind firmly to all B lymphocytes and may be used as reliable markers for these cells. We also suggest that the binding of Brucella species to B lymphocytes may have some bearing on the pathogenesis of brucellosis in humans.
在先前的研究中，我们已经表明，各种淋巴细胞亚群能结合不同属和种的不同菌株细菌。这些细菌中有一株羊种布鲁氏菌，它能与所有人类B淋巴细胞结合。为了确定羊种布鲁氏菌与人类B淋巴细胞的结合是菌株、种还是属的特征，我们测试了羊种布鲁氏菌、牛种布鲁氏菌、绵羊种布鲁氏菌、猪种布鲁氏菌、犬种布鲁氏菌和新墨西哥州布鲁氏菌与人类正常和白血病B淋巴细胞的结合情况。通过单标记和双标记实验确定不同布鲁氏菌物种与B淋巴细胞的结合，其中一株用抗轻链抗体包被的大肠杆菌用作B细胞的标记物。与先前的实验一样，我们发现羊种布鲁氏菌和抗体包被的大肠杆菌与相同的细胞结合。此外，我们发现所有测试的其他细菌物种都与正常或白血病B淋巴细胞结合。绵羊种布鲁氏菌和新墨西哥州布鲁氏菌不是人类病原体，它们与B淋巴细胞的结合比人类病原体牛种布鲁氏菌、羊种布鲁氏菌、猪种布鲁氏菌和犬种布鲁氏菌少。此外，我们发现非致病性细菌与淋巴细胞形成的玫瑰花结的质量，即每个淋巴细胞上的细菌数量，低于致病性布鲁氏菌物种。我们得出结论，所有测试的布鲁氏菌物种都有能力与人类B淋巴细胞结合，但只有那些人类病原体能牢固地与所有B淋巴细胞结合，并可作为这些细胞的可靠标记物。我们还认为，布鲁氏菌物种与B淋巴细胞的结合可能与人类布鲁氏菌病的发病机制有关。
