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淋病奈瑟菌感染研究。八、淋病奈瑟菌的125碘标记及其与真核细胞体外相互作用的研究。

Studies on gonococcus infection. VIII. 125Iodine labeling of gonococci and studies on their in vitro interactions with eukaryotic cells.

作者信息

Swanson J, King G, Zeligs B

出版信息

Infect Immun. 1975 Mar;11(3):453-9. doi: 10.1128/iai.11.3.453-459.1975.

DOI:10.1128/iai.11.3.453-459.1975
PMID:803927
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC415085/
Abstract

Intact gonococci (GC) have been labeled with 125iodine by the lactoperoxidase plus hydrogen peroxide procedure. The specific activities of types 2, 4, and 4 GC have been determined and are found to show small differences as follows: T4 greater than T2 greater than T4. 125I-labeled GC have been studied for their associations with both leukocytes and tissue culture cells. 125I-labeled GCshow the following relative order of association with the leukocytes: T2 equals T4* greater than T4. This contrasts with the relative degree of interaction between the GC and tissue culture cells, which follows the relative order: T2 greater than T4 equals T4*. Trypsin pretreatment of GC markedly reduces the association of all three types (T2, T4 AND T4*) with leukocytes but does not alter the level of attachment of any of the gonococcal types with tissue culture cells.

摘要

完整的淋球菌(GC)已通过乳过氧化物酶加过氧化氢法用125碘进行标记。已测定2型、4型和4型GC的比活性,发现它们存在如下小差异:T4大于T2大于T4。已研究了125I标记的GC与白细胞和组织培养细胞的结合情况。125I标记的GC与白细胞的结合呈现以下相对顺序:T2等于T4大于T4。这与GC和组织培养细胞之间的相互作用相对程度形成对比,后者遵循以下相对顺序:T2大于T4等于T4。用胰蛋白酶预处理GC可显著降低所有三种类型(T2、T4和T4*)与白细胞的结合,但不会改变任何淋球菌类型与组织培养细胞的附着水平。

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本文引用的文献

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Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes.酶促碘化作用。一种检测正常和肿瘤性淋巴细胞可及表面蛋白的探针。
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Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro.淋病奈瑟菌。二。35个月体外培养期间的菌落变异与致病性
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Studies on gonococcus infection. VI. Electron microscopic study on in vitro phagocytosis of gonococci by human leukocytes.淋病奈瑟菌感染的研究。VI. 人白细胞对淋病奈瑟菌体外吞噬作用的电子显微镜研究。
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Differential attachment by piliated and nonpiliated Neisseria gonorrhoeae to human sperm.有菌毛和无菌毛的淋病奈瑟菌对人类精子的差异黏附作用。
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