Swanson J, King G, Zeligs B
Infect Immun. 1975 Mar;11(3):453-9. doi: 10.1128/iai.11.3.453-459.1975.
Intact gonococci (GC) have been labeled with 125iodine by the lactoperoxidase plus hydrogen peroxide procedure. The specific activities of types 2, 4, and 4 GC have been determined and are found to show small differences as follows: T4 greater than T2 greater than T4. 125I-labeled GC have been studied for their associations with both leukocytes and tissue culture cells. 125I-labeled GCshow the following relative order of association with the leukocytes: T2 equals T4* greater than T4. This contrasts with the relative degree of interaction between the GC and tissue culture cells, which follows the relative order: T2 greater than T4 equals T4*. Trypsin pretreatment of GC markedly reduces the association of all three types (T2, T4 AND T4*) with leukocytes but does not alter the level of attachment of any of the gonococcal types with tissue culture cells.
完整的淋球菌(GC)已通过乳过氧化物酶加过氧化氢法用125碘进行标记。已测定2型、4型和4型GC的比活性,发现它们存在如下小差异:T4大于T2大于T4。已研究了125I标记的GC与白细胞和组织培养细胞的结合情况。125I标记的GC与白细胞的结合呈现以下相对顺序:T2等于T4大于T4。这与GC和组织培养细胞之间的相互作用相对程度形成对比,后者遵循以下相对顺序:T2大于T4等于T4。用胰蛋白酶预处理GC可显著降低所有三种类型(T2、T4和T4*)与白细胞的结合,但不会改变任何淋球菌类型与组织培养细胞的附着水平。