5-甲氧基尿苷：一种新的次要成分，位于枯草芽孢杆菌tRNAAla、tRNAThr和tRNAVal反密码子的第一位。
5-methoxyuridine: a new minor constituent located in the first position of the anticodon of tRNAAla, tRNAThr, and tRNAVal from Bacillus subtilis.
作者信息
Murao K, Hasegawa T, Ishikura H
出版信息
Nucleic Acids Res. 1976 Oct;3(10):2851-60. doi: 10.1093/nar/3.10.2851.
Abstract
The sequences of the anticodon of tRNAAla, tRNAThr, and tRNAVal from Bacillus subtilis W 168 were N-G-C, N-G-U, and N-A-C, respectively. A new minor constituent, N, occupied the first position of the anticodon of each tRNA. N was indentified as 5-methoxyuridine (mo5U, Figure 1) by comparison of its UV absorption spectra, Rf values in thin-layer chromatography using several solvent systems and mass spectra with those of chemically synthesized specimen.
摘要
来自枯草芽孢杆菌W168的tRNAAla、tRNAThr和tRNAVal反密码子序列分别为N-G-C、N-G-U和N-A-C。一种新的次要成分N占据了每种tRNA反密码子的第一位。通过比较其紫外吸收光谱、在几种溶剂系统中的薄层色谱Rf值和质谱与化学合成样品的相应值，N被鉴定为5-甲氧基尿苷（mo5U，图1）。
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