大肠杆菌K-12中L-丝氨酸的降解：直接分离的ssd突变体及其基因内回复突变体
L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants.
作者信息
Newman E B, Malik N, Walker C
出版信息
J Bacteriol. 1982 May;150(2):710-5. doi: 10.1128/jb.150.2.710-715.1982.
Abstract
Two methods for the direct isolation of spontaneous ssd mutants of Escherichia coli K-12 strains are described; (i) by growth with L-serine as the carbon source, and (ii) by low-level kanamycin resistance. A newly isolated mutant had the same phenotype as the mutant described previously, including inefficient use of the glucose, inability to growth with succinate, altered transport characteristics, and altered resistance to certain growth effectors. Succinate-utilizing derivatives which appear to be intragenic are characterized in detail. The relation between the mutants isolated here and mutants which are thought to have impairment in a system of coupling respiratory energy to active transport (ecfB mutants) is discussed.
摘要
本文描述了两种直接分离大肠杆菌K-12菌株自发ssd突变体的方法；（i）以L-丝氨酸作为碳源进行培养，以及（ii）通过低水平卡那霉素抗性筛选。新分离出的一个突变体具有与先前描述的突变体相同的表型，包括对葡萄糖利用效率低下、无法利用琥珀酸生长、转运特性改变以及对某些生长效应物的抗性改变。详细表征了似乎是基因内的琥珀酸利用衍生物。讨论了此处分离的突变体与被认为在呼吸能量与主动转运偶联系统中存在缺陷的突变体（ecfB突变体）之间的关系。
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