一种检测人内源性（白细胞）致热原的新的灵敏方法。

A new sensitive method for detecting human endogenous (leukocyte) pyrogen.

作者信息

Bodel P, Miller H

出版信息

Inflammation. 1978 Mar;3(1):103-10. doi: 10.1007/BF00917325.

Abstract

Endogenous, or leukocyte pyrogen (EP), the mediator of fever, is currently detected by injection of pyrogen-containing supernatants into rabbits. This assay has been of little value in the study of human fever because it required injection of relatively large amounts of pyrogen. We now report that injection of medium containing human EP produces fever in mice. Supernatant from 1 c 10(5) granulocytes, stimulated by phagocytosis of staphylococci and incubated overnight, or 1 x 10(4) monocytes similarly treated, produce clear pyrogenic responses. This method for detecting EP is about 100-fold more sensitive than the rabbit assay, and it appears to be specific for EP. Preliminary studies of EP released by small samples of needle liver biopsies from febrile and afebrile patients suggests that this sensitive assay may be useful for investigations into the mechanisms of clinical fever.

摘要

内源性致热原，即白细胞致热原（EP），作为发热的介质，目前是通过将含有致热原的上清液注射到兔子体内来检测的。该检测方法在人类发热研究中价值不大，因为它需要注射相对大量的致热原。我们现在报告，注射含有人类EP的培养基可使小鼠发热。由吞噬葡萄球菌刺激并孵育过夜的1×10⁵粒细胞的上清液，或经同样处理的1×10⁴单核细胞的上清液，会产生明显的致热反应。这种检测EP的方法比兔子检测法敏感约100倍，并且似乎对EP具有特异性。对发热和不发热患者的少量肝脏穿刺活检样本释放的EP进行的初步研究表明，这种灵敏的检测方法可能有助于临床发热机制的研究。