从酵母细胞质核糖体中分离出17种蛋白质并测定8种蛋白质的氨基末端氨基酸序列。

Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast.

作者信息

Otaka E, Higo K, Osawa S

出版信息

Biochemistry. 1982 Sep 14;21(19):4545-50. doi: 10.1021/bi00262a005.

Abstract

Seventeen ribosomal proteins of Saccharomyces cerevisiae were isolated from small ribosomal subunits and disodium ethylenediaminetetraacetate treated 80S ribosomes by chromatography on a column of carboxymethylcellulose and/or by filtration through Sephacryl S-200. The isolated proteins are YS4, YS7, YS8, YS9, YS10, YS12, YS14, YS18, YS23, YS29, YL11, YL13, YL16, YL17, YL22, YL38, and YL40 [nomenclature according to Otaka & Osawa (1981) [Otaka, E., & Osawa, S. (1981) Mol. Gen. Genet. 181, 176-182]]. The purification procedures and the amino acid compositions of these proteins are presented. Amino-terminal amino acid sequences of YS4, YS6, YS11, YS15, YS16, YS22, YL10, and YL31 have been determined and compared with those from rat liver [Wittmann-Liebold, B., Geissler, A. W., Lin, A., & Wool, I. G. (1979) J. Supramol. Struct. 12, 425-433] and Halobacterium cutirubrum [Matheson, A. T., Möller, W., Amons, R., & Yaguchi, M. (1980) in Ribosomes: Structure, Function and Genetics (Chambliss, G., et al., Eds.) pp 297-332, University Park Press, Baltimore, MD; M. Yaguchi, unpublished experiments].

摘要

从酿酒酵母的小核糖体亚基以及经乙二胺四乙酸二钠处理的80S核糖体中，通过羧甲基纤维素柱层析和/或经Sephacryl S - 200过滤，分离出了17种核糖体蛋白。分离出的蛋白为YS4、YS7、YS8、YS9、YS10、YS12、YS14、YS18、YS23、YS29、YL11、YL13、YL16、YL17、YL22、YL38和YL40[命名依据Otaka和Osawa（1981年）[Otaka, E., & Osawa, S. (1981) Mol. Gen. Genet. 181, 176 - 182]]。文中展示了这些蛋白的纯化步骤和氨基酸组成。已测定了YS4、YS6、YS11、YS15、YS16、YS22、YL10和YL31的氨基末端氨基酸序列，并与大鼠肝脏[Wittmann - Liebold, B., Geissler, A. W., Lin, A., & Wool, I. G. (1979) J. Supramol. Struct. 12, 425 - 433]以及红皮盐杆菌[Matheson, A. T., Möller, W., Amons, R., & Yaguchi, M. (1980) in Ribosomes: Structure, Function and Genetics (Chambliss, G., et al., Eds.) pp 297 - 332, University Park Press, Baltimore, MD; M. Yaguchi, unpublished experiments]的相应序列进行了比较。