Wilson M C, Harvey J D
Biophys J. 1983 Jan;41(1):13-21. doi: 10.1016/S0006-3495(83)84400-2.
Previous laser light-scattering studies of spermatozoon motility have been hampered by the large, asymmetric shape of spermatozoa, which causes difficulties in the interpretation of intensity fluctuations in the light scattered from a single laser beam. This paper describes an experimental arrangement for measuring the distribution of transit times for swimming spermatozoa using two slightly separated, focused laser beams. The theory of operation of the instrument is developed to enable the analysis of the experimentally obtained cross-correlation functions. The effects of the pronounced spermatozoon asymmetry and associated intensity modulation in the scattered light are also investigated and shown to be negligible for the twin beam experimental arrangement, provided that the swimming speed distribution has a coefficient of variation (sigma/upsilon greater than 0.1. Results obtained using this apparatus are presented for the velocity distribution of spermatozoa from a variety of bulls.
以往对精子活力的激光光散射研究因精子大且不对称的形状而受阻，这给解释从单个激光束散射的光的强度波动带来困难。本文描述了一种使用两个稍微分开的聚焦激光束来测量游动精子通过时间分布的实验装置。对该仪器的工作原理进行了深入研究，以便能够分析实验获得的互相关函数。还研究了精子明显不对称以及散射光中相关强度调制的影响，并表明对于双光束实验装置来说这些影响可忽略不计，前提是游动速度分布的变异系数（σ/υ大于0.1）。展示了使用该仪器获得的来自多种公牛精子的速度分布结果。
