Craig T, Hallett F R
Biophys J. 1982 Apr;38(1):71-8. doi: 10.1016/S0006-3495(82)84532-3.
The half-width scaling of experimental and model electric field autocorrelation functions of light scattered from normally swimming, defectively swimming, and immotile bull spermatozoa is examined. It is found that a scatter of size 9.0 x 2.3 x 0.45 micrometers is most appropriate for this Rayleigh-Gans-Debye ellipsoid model. In the case of the immotile cells, this model correctly predicts the features seen in the scaling data as well as the absolute value of the data. For the normally swimming and defective populations the model proves to predict correctly the features seen in experimental scaling curves, but not the absolute value of the data. This discrepancy appears to be related to a lack of detail in the model, since the agreement is poorest at large scattering angles.
研究了从正常游动、游动缺陷和不游动的公牛精子散射的光的实验和模型电场自相关函数的半高宽缩放。发现尺寸为9.0×2.3×0.45微米的散射体最适合该瑞利 - 甘斯 - 德拜椭球模型。对于不游动的细胞，该模型正确地预测了缩放数据中看到的特征以及数据的绝对值。对于正常游动和有缺陷的群体，该模型被证明能够正确预测实验缩放曲线中看到的特征，但不能预测数据的绝对值。这种差异似乎与模型中缺乏细节有关，因为在大散射角处一致性最差。
