从活动微生物散射的激光的光谱分析。
Spectral analysis of laser light scattered from motile microorganisms.
作者信息
Nossal R
出版信息
Biophys J. 1971 Apr;11(4):341-54. doi: 10.1016/S0006-3495(71)86219-7.
Abstract
The theoretical basis of laser scattering from motile microorganisms is examined. Spectra of swimming particles are compared with spectra arising from brownian motion. For mixtures of motile and resting organisms, that part of the spectrum related to the motile organisms is enhanced when V(s)/|k|D is large, where V(s) is the mean swimming speed of the motile microorganisms,|k| is the Bragg wave vector, and D is the diffusion coefficient of the nonmotile particles. When the directed motion of swimming microorganisms persists for periods which are much longer than tau = (|k|V(s))(-1), the scattering spectrum is given as S(k, omega) infinity P(| [omega - omega(0)]/k|), where P is the probability distribution obtained by two-dimensional integration over the swimming speed distribution. A computation of scattering from bull spermatozoa, based on published velocity distributions, is investigated in detail.
摘要
研究了运动微生物激光散射的理论基础。将游泳颗粒的光谱与布朗运动产生的光谱进行了比较。对于运动和静止生物体的混合物，当V(s)/|k|D很大时，与运动生物体相关的光谱部分会增强，其中V(s)是运动微生物的平均游泳速度，|k|是布拉格波矢，D是非运动颗粒的扩散系数。当游泳微生物的定向运动持续的时间远长于τ = (|k|V(s))(-1)时，散射光谱表示为S(k, ω) ∞ P(| [ω - ω(0)]/k|)，其中P是通过对游泳速度分布进行二维积分得到的概率分布。基于已发表的速度分布，详细研究了公牛精子散射的计算。
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