Nakata S, Chiba S, Terashima H, Sakuma Y, Kogasaka R, Nakao T
J Clin Microbiol. 1983 Feb;17(2):198-201. doi: 10.1128/jcm.17.2.198-201.1983.
A microtiter solid-phase radioimmunoassay (RIA) was developed for detection of human calicivirus in stool specimens. Seventy-eight stool specimens were tested by RIA. All 17 specimens positive for human calicivirus by electron microscopy (EM) were also positive by RIA. In addition, of 21 specimens obtained from an outbreak of caliciviral gastroenteritis, 11 were positive by RIA but negative by EM. Of 20 specimens positive for rotavirus by EM and 20 nondiarrheic specimens with no virus, 2 and 1, respectively, were positive by RIA but were subsequently shown to be falsely positive by a blocking test. There was no cross-reaction between human and feline caliciviruses. Thus, the test was more sensitive than EM and, with an appropriate blocking test, was specific for human calicivirus. It might be especially useful for screening large numbers of stool specimens.
开发了一种微量滴定板固相放射免疫测定法（RIA），用于检测粪便标本中的人杯状病毒。用RIA检测了78份粪便标本。通过电子显微镜（EM）检测为人杯状病毒阳性的所有17份标本，用RIA检测也呈阳性。此外，在从一次杯状病毒性肠胃炎暴发中获得的21份标本中，11份用RIA检测呈阳性，但用EM检测呈阴性。在通过EM检测为轮状病毒阳性的20份标本和20份无病毒的非腹泻标本中，分别有2份和1份用RIA检测呈阳性，但随后通过阻断试验显示为假阳性。人杯状病毒和猫杯状病毒之间没有交叉反应。因此，该检测方法比EM更敏感，并且通过适当的阻断试验，对人杯状病毒具有特异性。它可能对筛查大量粪便标本特别有用。
