Adams D, Atkinson H M, Woods W H
J Clin Microbiol. 1983 Mar;17(3):422-7. doi: 10.1128/jcm.17.3.422-427.1983.
An agglutination typing scheme has been developed for strains of Aeromonas hydrophila. Primary agglutination typing is based on testing agar-grown A. hydrophila cells with human, horse, rat, and guinea pig erythrocytes and Saccharomyces cerevisiae cells. Further subdivision of primary groups is based firstly on whether yeast cell agglutination is inhibited by a D-mannose polymer, yeast mannan, and secondly on patterns of inhibition of hemagglutination by yeast mannan and the monomeric sugars L-fucose, D-galactose, and D-mannose. A total of 320 isolates were tested, and these were divisible into 39 distinct types on the basis of this scheme. Application of this typing scheme in the future to isolates of A. hydrophila known to be associated with human infection may enable correlations to be made between particular agglutination types and human pathogenicity.
已开发出一种针对嗜水气单胞菌菌株的凝集分型方案。初步凝集分型基于用人、马、大鼠和豚鼠红细胞以及酿酒酵母细胞对琼脂培养的嗜水气单胞菌细胞进行检测。主要菌群的进一步细分首先基于酵母细胞凝集是否被D - 甘露糖聚合物、酵母甘露聚糖抑制,其次基于酵母甘露聚糖以及单体糖L - 岩藻糖、D - 半乳糖和D - 甘露糖对血凝的抑制模式。共检测了320株分离株,根据该方案可将它们分为39种不同类型。未来将此分型方案应用于已知与人类感染相关的嗜水气单胞菌分离株,可能有助于建立特定凝集类型与人类致病性之间的关联。