Adam E, Peel S
J Anat. 1983 Mar;136(Pt 2):321-8.
Explants of metrial gland were taken from uteri of rats at day 13 of pregnancy and maintained in vitro for up to 9 days. Some cultures were supplemented with progesterone. The progress of the explants was monitored by light and electron microscopy. In the absence of progesterone, large amounts of collagen were apparent and many cells contained lipid inclusions. The granulated cells which are characteristic of the gland were not seen after 4 days in culture. In the presence of progesterone, though apparently normal granulated cells were readily found after 4 days in culture and could be identified after 9 days, some showed changes which do not occur in vivo. Collagen accumulation and the development of lipid inclusions were less extensive in the presence of progesterone.
在妊娠第13天从大鼠子宫中取出蜕膜腺外植体，并在体外培养长达9天。一些培养物添加了孕酮。通过光学显微镜和电子显微镜监测外植体的进展。在没有孕酮的情况下，大量胶原蛋白明显可见，许多细胞含有脂质包涵体。培养4天后未见到该腺体特有的颗粒细胞。在有孕酮的情况下，虽然培养4天后很容易发现明显正常的颗粒细胞，并且在9天后可以识别，但有些细胞显示出在体内不会出现的变化。在有孕酮的情况下，胶原蛋白积累和脂质包涵体的形成程度较轻。
