Coates C L, Burwell R G, Lloyd-Jones K, Swannell A J, Walker G, Selby C
Ann Rheum Dis. 1978 Aug;37(4):303-14. doi: 10.1136/ard.37.4.303.
The somatomedin activity in synovial fluids from 50 patients with a variety of joint diseases has been studied and compared with the activity in each of the patient's own serum and a standard reference serum (SRS). The porcine costal cartilage bioassay of Van den Brande and Du Caju (1974a) has been used with the isotopes 3H-thymidine and 35S-sulphate. Synovial fluids from most patients with post-traumatic and post-operative effusions, osteoarthritis and arthritis associated with psoriasis, Reiter's disease, and ankylosing spondylitis stimulated the synthesis of DNA and proteoglycans in cartilage. Synovial fluids from patients with rheumatoid arthritis either had impaired capacity to stimulate DNA synthesis, or they inhibited it; a similar, but less evident pattern was observed for proteoglycan synthesis. Some synovial fluids from patients with miscellaneous synovitides stimulated, while others inhibited cartilage metabolism. It is concluded that the synovial fluid from patients with rheumatoid arthritis and from some patients with miscellaneous synovitides contained an inhibitor(s) to DNA and possibly proteoglycan synthesis. The sera from nearly all the patients stimulated both DNA and proteoglycan synthesis, but the somatomedin potency ratios for serum in terms of SRS were generally less than 1.0. There was a significant inverse correlation between the serum somatomedin potency ratio and the age of the patient.
对50例患有各种关节疾病患者的滑液中的生长调节素活性进行了研究，并与每位患者自身血清及标准参考血清（SRS）中的活性进行了比较。采用了Van den Brande和Du Caju（1974a）的猪肋软骨生物测定法，使用了同位素³H - 胸腺嘧啶核苷和³⁵S - 硫酸盐。大多数创伤后和术后积液、骨关节炎以及与银屑病、赖特综合征和强直性脊柱炎相关的关节炎患者的滑液刺激了软骨中DNA和蛋白聚糖的合成。类风湿关节炎患者的滑液要么刺激DNA合成的能力受损，要么抑制DNA合成；在蛋白聚糖合成方面观察到类似但不太明显的模式。一些患有其他滑膜炎患者的滑液刺激了软骨代谢，而另一些则抑制了软骨代谢。得出的结论是，类风湿关节炎患者和一些患有其他滑膜炎患者的滑液中含有DNA和可能的蛋白聚糖合成抑制剂。几乎所有患者的血清都刺激了DNA和蛋白聚糖的合成，但血清相对于SRS的生长调节素效价比通常小于1.0。血清生长调节素效价比与患者年龄之间存在显著的负相关。
