Coates C L, Burwell R G, Buttery P J, Walker G, Woodward P M
Ann Rheum Dis. 1977 Feb;36(1):50-5. doi: 10.1136/ard.36.1.50.
Abnormalities of synovial fluid, as a lubricant and nutrient, may have relevance to the causation of certain articular diseases. The somatomedin activity in normal synovial fluid obtained from the knee joint of the ox has been studied and compared with the activity in serum from the same animal. The porcine costal cartilage bioassay of Van den Brande and Du Caju (1974) has been used with the isotopes 35S-sulphate and 3H-thymidine. The mean potency ratio of ox synovial fluid in terms of ox serum for 35S-sulphate incorporation was 0-28 (range 0-19-0-47) and for 3H-thymidine incorporation 0-35 (range 0-21-0-63). A significant correlation was found between the somatomedin activity (as measured by 35S-sulphate incorporation) and the total protein and albumin concentrations in the ox synovial fluids and the ox sera, but there was no significant relationship between the somatomedin potency ratios and the globulin concentrations. The possible relevance of these findings to injury and disease in synovial joint is discussed.
作为润滑剂和营养物质，滑液异常可能与某些关节疾病的病因有关。对从牛膝关节获取的正常滑液中的生长调节素活性进行了研究，并与同一动物血清中的活性进行了比较。采用了范登布兰德和杜卡朱（1974年）的猪肋软骨生物测定法，使用了同位素35S-硫酸盐和3H-胸腺嘧啶核苷。就35S-硫酸盐掺入而言，牛滑液相对于牛血清的平均效价比为0.28（范围为0.19 - 0.47），就3H-胸腺嘧啶核苷掺入而言为0.35（范围为0.21 - 0.63）。在牛滑液和牛血清中，生长调节素活性（通过35S-硫酸盐掺入测定）与总蛋白和白蛋白浓度之间存在显著相关性，但生长调节素效价比与球蛋白浓度之间无显著关系。讨论了这些发现与滑膜关节损伤和疾病的可能相关性。
