Fredericks C M, Lundquist L E, Mathur R S, Ashton S H, Landgrebe S C
Biol Reprod. 1983 Jun;28(5):1052-60. doi: 10.1095/biolreprod28.5.1052.
The purpose of this study was to determine the effects of infused vasoactive intestinal polypeptide (VIP) upon reproductive function in the female rabbit. Intravenous infusions of VIP (37.5, 75, and 150 pmol/kg per min) induced acute dose-dependent increases in plasma progesterone (P) but not estradiol (E2) or testosterone (T) in estrous rabbits. This P effect was not associated with an increase in plasma prolactin (Prl) and was not altered by pretreatment with a Prl-inhibiting regimen of bromocriptine. In rabbits stimulated to ovulate with 75 IU human chorionic gonadotropin (hCG) and coitus, plasma P and E2 increased, reaching a peak at 180 min following stimulation. VIP (75 pmol/kg per min) infused from 120 to 180 min following the ovulatory stimuli increased this P peak but did not effect E2 levels. This VIP infusion had no effect upon fertility or upon the number of corpora lutea, uterine implants, or viable conceptuses. Infusions of VIP for 60 min at the P peak, and for 240 min at the time of ovulation, had no significant effect upon ovum pickup or the rate of ovum transport. These observations suggest that 1) VIP infusions in rabbits can increase plasma P from both the basal levels of estrus and from the peak levels preceding ovluation. 2) Infusions of VIP at the time of the preovulatory steroid surge or during ovulation have little effect upon fertility or gamete transport in the rabbit. 3) Endogenous VIP may play a role in the regulation of P secretion in the rabbit.
本研究的目的是确定静脉输注血管活性肠肽（VIP）对雌性兔生殖功能的影响。对处于发情期的兔静脉输注VIP（37.5、75和150 pmol/kg每分钟）可引起血浆孕酮（P）呈急性剂量依赖性升高，但雌二醇（E2）或睾酮（T）无变化。这种孕酮效应与血浆催乳素（Prl）升高无关，且用溴隐亭的催乳素抑制方案预处理后也未改变。在用75 IU人绒毛膜促性腺激素（hCG）刺激排卵并进行交配的兔中，血浆P和E2升高，在刺激后180分钟达到峰值。在排卵刺激后120至180分钟输注VIP（75 pmol/kg每分钟）可使该孕酮峰值升高，但不影响E2水平。这种VIP输注对生育力或黄体数量、子宫着床或存活胚胎数量均无影响。在孕酮峰值时输注VIP 60分钟，以及在排卵时输注240分钟，对卵子采集或卵子运输速率均无显著影响。这些观察结果表明：1）兔静脉输注VIP可使发情期基础水平以及排卵前峰值水平的血浆P均升高。2）在排卵前类固醇激增时或排卵期间输注VIP对兔的生育力或配子运输影响很小。3）内源性VIP可能在兔孕酮分泌的调节中起作用。
