Nigro N D, Campbell R L
Cancer Lett. 1978 Aug;5(2):91-5. doi: 10.1016/s0304-3835(78)80050-0.
Sprague--Dawley rats, both intact and colostomized animals, were given 24 weekly injections of azoxymethane. Rats were fed either Rat Purina Chow or the same diet plus 0.25% disulfiram. In the intact animals, disulfiram reduced tumors from an average of 6.3 to 0.95. The number of rats developing tumors was reduced from 100% to 60%. In colostomized animals, the reduction was from an average of 5.0 to 0.13. Marked inhibition occurred even in the defunctionalized colon. The results suggest that disulfiram blocks the metabolism of azoxymethane to methylazoxymethanol, and also that the inhibitor may act systemically.
对斯普拉格-道利大鼠，包括未做处理的和做了结肠造口术的动物，每周注射一次偶氮甲烷，共注射24次。给大鼠喂食普瑞纳大鼠饲料或相同饲料加0.25%的双硫仑。在未做处理的动物中，双硫仑使肿瘤数量从平均6.3个减少到0.95个。发生肿瘤的大鼠数量从100%减少到60%。在做了结肠造口术的动物中，肿瘤数量从平均5.0个减少到0.13个。即使在功能缺失的结肠中也出现了明显的抑制作用。结果表明，双硫仑可阻断偶氮甲烷代谢为甲基偶氮甲醇，而且这种抑制剂可能具有全身作用。
