泼尼松龙对溃疡性结肠炎直肠黏膜前列腺素合成的影响：通过层流生物测定法和放射免疫测定法进行的研究。

Effect of prednisolone on prostaglandin synthesis by rectal mucosa in ulcerative colitis: investigation by laminar flow bioassay and radioimmunoassay.

作者信息

Hawkey C J, Truelove S C

出版信息

Gut. 1981 Mar;22(3):190-3. doi: 10.1136/gut.22.3.190.

DOI:10.1136/gut.22.3.190

PMID:7227851

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1419508/

Abstract

The effect of two concentrations of prednisolone on synthesis of prostaglandin E2 (PGE2) by 40 rectal biopsies in organ culture was investigated using both laminar flow bioassay and radioimmunoassay (RIA). Prednisolone (concentration 8.33 x 10(-7)M) reduced mean synthesis of PGE2 to 36.4% of control values (measured by bioassay) or 26.2% of control values (measured by RIA). With prednisolone (concentration 5.66 X 10(-4) M) synthesis of PGE2 was 7.7% of control values (RIA). The two concentrations are similar respectively to those achieved in plasma after oral prednisolone and delivered topically by prednisolone enemata. Inhibition of PG synthesis may thus explain prednisolone's anti-inflammatory action in the treatment of ulcerative colitis.

摘要

采用层流生物测定法和放射免疫测定法（RIA），研究了两种浓度的泼尼松龙对40份直肠活检组织在器官培养中前列腺素E2（PGE2）合成的影响。泼尼松龙（浓度8.33×10⁻⁷M）使PGE2的平均合成量降至对照值的36.4%（通过生物测定法测量）或对照值的26.2%（通过放射免疫测定法测量）。对于泼尼松龙（浓度5.66×10⁻⁴M），PGE2的合成量为对照值的7.7%（放射免疫测定法）。这两种浓度分别与口服泼尼松龙后血浆中达到的浓度以及通过泼尼松龙灌肠剂局部给药所达到的浓度相似。因此，PG合成的抑制可能解释了泼尼松龙在治疗溃疡性结肠炎中的抗炎作用。