用于检测奈瑟菌属和卡他布兰汉菌利用碳水化合物产酸的改良氧化发酵培养基。
Modified oxidation-fermentation medium for detection of acid production from carbohydrates by Neisseria spp. and Branhamella catarrhalis.
作者信息
Knapp J S, Holmes K K
出版信息
J Clin Microbiol. 1983 Jul;18(1):56-62. doi: 10.1128/jcm.18.1.56-62.1983.
Abstract
A modified oxidation-fermentation medium was developed as a practical medium for highly sensitive and specific detection of acid production from carbohydrates by Neisseria spp. and Branhamella catarrhalis. A total of 756 strains representing 17 Neisseria spp. and Branhamella catarrhalis were tested in this medium, in which the protein concentration was reduced relative to the carbohydrate concentration, phenol red was substituted for bromthymol blue at a low concentration, and the initial pH was adjusted to 7.2. Sugar utilization patterns were consistent with published results and with other cultural and biochemical characteristics for these species. The reactions obtained using this medium were qualitatively better and more reproducible than those obtained in cystine-Trypticase agar (BBL Microbiology Systems, Cockeysville, Md.) medium.
摘要
一种改良的氧化发酵培养基被开发出来，作为一种实用培养基，用于高度灵敏且特异的检测奈瑟菌属和卡他布兰汉菌利用碳水化合物产酸的情况。总共756株代表17种奈瑟菌属和卡他布兰汉菌的菌株在该培养基中进行了测试，该培养基中蛋白质浓度相对于碳水化合物浓度降低，低浓度的酚红替代了溴麝香草酚蓝，初始pH值调至7.2。糖利用模式与已发表结果以及这些菌种的其他培养和生化特征相符。使用该培养基获得的反应在质量上比在胱氨酸胰蛋白酶琼脂（BBL微生物系统公司，马里兰州科基斯维尔）培养基中获得的反应更好且更具重复性。
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