Denburg J A, Wilson W E, Goodacre R, Bienenstock J
Br J Haematol. 1980 May;45(1):13-21. doi: 10.1111/j.1365-2141.1980.tb03806.x.
We have attempted to assess basophil differentiation in vitro in 15 patients with chronic myeloid leukaemia (CML). Using a sensitive radioassay, whole blood histamine values were found to be elevated in 10 of 14 patients tested, and correlated well with peripheral blood basophil counts (r=¿70). In seven of nine CML patients, but not in controls, total histamine content of separated peripheral blood cells suspended in a modified Marbrook system was shown to rise after 7 d in vitro. Further study showed that both histamine (mean, five-fold increase) and basophils (mean, three-fold increase) were significantly elevated over control values at 1, 2 and 3 weeks in vitro. Total cell-associated histamine content (in pg per 100 viable cells) was greater in CML cultures than in controls at 3 weeks (P less than 0.01). Serial cultures of cells from one patient revealed substantial in vitro rises in basophils and histamine at an accelerated, but not at a chronic, phase of disease. Cells from this patient and two others studied at the time of blast crisis demonstrated higher indices of basophil and histamine increases when compared to the group of CML patients in chronic phases of the disease (P less than 0.05). We conclude that basophil precursors exist in increased numbers in the peripheral blood of CML patients. Assays for basophil differentiation may prove useful in following disease activity in this and other myeloproliferative disorders.
我们试图评估15例慢性粒细胞白血病（CML）患者的嗜碱性粒细胞体外分化情况。使用灵敏的放射分析方法，在14例接受检测的患者中，有10例全血组胺值升高，且与外周血嗜碱性粒细胞计数相关性良好（r = 0.70）。在9例CML患者中的7例（而非对照组），悬浮于改良Marbrook系统中的分离外周血细胞总组胺含量在体外培养7天后显示升高。进一步研究表明，在体外培养1、2和3周时，组胺（平均升高5倍）和嗜碱性粒细胞（平均升高3倍）均显著高于对照值。在3周时，CML培养物中每100个活细胞的总细胞相关组胺含量（以皮克计）高于对照组（P小于0.01）。对一名患者的细胞进行连续培养显示，在疾病的加速期而非慢性期，嗜碱性粒细胞和组胺在体外有显著升高。与处于疾病慢性期的CML患者组相比，该患者以及另外两名在原始细胞危象时进行研究的患者的细胞，嗜碱性粒细胞和组胺升高指数更高（P小于0.05）。我们得出结论，CML患者外周血中嗜碱性粒细胞前体数量增加。嗜碱性粒细胞分化检测可能对监测该疾病及其他骨髓增殖性疾病的疾病活动有用。
