Lindqvist L, Otteskog P
Scand J Dent Res. 1980 Dec;88(6):552-6. doi: 10.1111/j.1600-0722.1980.tb01266.x.
The amount of eugenol (4-allyl-2-methoxyphenol) released from four different dental materials immersed in phosphate buffer was measured by gas liquid chromatography. Maximal release of eugenol from zinc oxide-eugenol cement (ZOE) and IRM was attained within 5 h and corresponded to 5% and 4%, respectively, of the total amount of eugenol in each material. Both the rates and total amounts of eugenol released from Nobetec and Opotow were lower than for ZOE and IRM. Eugenol (0.67 mM) applied to growing cultures of human diploid fibroblasts reduced the number of cells recovered to a value which was 4% of that found for control cultures which grew to form monolayers. Confluent monolayers of 3H-uridine labeled cell cultures which were incubated for 1 h in the presence of 4 mM eugenol lost approximately 100% of this cytoplasmic label, indicating total cell death.
通过气液色谱法测定了四种不同牙科材料浸泡在磷酸盐缓冲液中丁香酚（4-烯丙基-2-甲氧基苯酚）的释放量。氧化锌丁香酚水门汀（ZOE）和IRM中丁香酚的最大释放量在5小时内达到，分别相当于每种材料中丁香酚总量的5%和4%。Nobetec和Opotow释放丁香酚的速率和总量均低于ZOE和IRM。将丁香酚（0.67 mM）应用于人类二倍体成纤维细胞的生长培养物中，使回收的细胞数量降至对照培养物（生长形成单层）的4%。在4 mM丁香酚存在下孵育1小时的3H-尿苷标记细胞培养物的汇合单层失去了约100%的这种细胞质标记，表明细胞全部死亡。
