神经胶质成熟因子促进癌细胞的接触抑制。

Lim R, Nakagawa S, Arnason B G, Turriff D E

Proc Natl Acad Sci U S A. 1981 Jul;78(7):4373-7. doi: 10.1073/pnas.78.7.4373.

The effect of bovine glia maturation factor on the growth pattern of cancer cells was investigated in the rat glioma cell line 354A. When the cells were grown in the serum-free defined medium N2 in the absence of the factor, the cells proliferated with a doubling time of 24 hr without showing contact inhibition. After reaching confluency, the cell layer formed numerous foci from which heaps of cell colonies arose. The addition of glia maturation factor to the culture stimulated cell division in the logarithmic phase but prevented overgrowth once the cells arrived at confluency. The ability of glia maturation factor to restore contact inhibition suggests a regulatory role in normal and neoplastic cells.

在大鼠胶质瘤细胞系354A中研究了牛胶质细胞成熟因子对癌细胞生长模式的影响。当细胞在无血清限定培养基N2中生长且无该因子时，细胞以24小时的倍增时间增殖，且未表现出接触抑制。达到汇合后，细胞层形成许多病灶，从中出现成堆的细胞集落。向培养物中添加胶质细胞成熟因子在对数期刺激细胞分裂，但一旦细胞达到汇合就会阻止过度生长。胶质细胞成熟因子恢复接触抑制的能力表明其在正常细胞和肿瘤细胞中具有调节作用。