用于新月柄杆菌的改良广义转导噬菌体。
Improved generalized transducing bacteriophage for Caulobacter crescentus.
作者信息
Bender R A
出版信息
J Bacteriol. 1981 Nov;148(2):734-5. doi: 10.1128/jb.148.2.734-735.1981.
Abstract
Caulobacter phage phi Cr30T, a temperature-sensitive derivative of the lytic, generalized transducing phage phi Cr30, was isolated as a double temperature-sensitive recombinant in a cross between phi Cr30ts1 and phi Cr30ts2, phi Cr30T mediated generalized transduction of Caulobacter crescentus at frequencies comparable to those of phi Cr30 and eliminated the requirement for irradiation of transducing lysates to prevent killing of transductants on the plate.
摘要
柄杆菌噬菌体φCr30T是裂解性、普遍性转导噬菌体φCr30的温度敏感衍生物，它是在φCr30ts1和φCr30ts2的杂交中作为双温度敏感重组体分离得到的。φCr30T介导新月柄杆菌的普遍性转导，其频率与φCr30相当，并且消除了对转导裂解物进行辐照以防止平板上的转导子死亡的需求。
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