一种18200道尔顿的肾上腺蛋白，含有四个甲硫氨酸脑啡肽序列。
An 18,200-dalton adrenal protein that contains four [Met]enkephalin sequences.
作者信息
Kilpatrick D L, Jones B N, Lewis R V, Stern A S, Kojima K, Shively J E, Udenfriend S
出版信息
Proc Natl Acad Sci U S A. 1982 May;79(9):3057-61. doi: 10.1073/pnas.79.9.3057.
Abstract
An 18,200-dalton adrenal enkephalin-containing polypeptide was purified to homogeneity and shown to contain four internal [Met]enkephalin sequences. Protein sequence analysis and mapping of trypsin-derived peptides demonstrated that the previously described 12,600-dalton and 5,300-dalton adrenal enkephalin-containing polypeptides compose the amino-terminal and carboxyl-terminal sequences, respectively, of this larger protein. They are linked by two basic amino acid residues to form the entire sequence of the 18,200-dalton protein. This protein is the largest enkephalin-containing polypeptide yet isolated from beef adrenal medulla and serves as the precursor for several other previously characterized adrenal enkephalin-containing proteins and peptides.
摘要
一种分子量为18200道尔顿、含肾上腺脑啡肽的多肽被纯化至同质，并显示含有四个内部[甲硫氨酸]脑啡肽序列。蛋白质序列分析以及对胰蛋白酶衍生肽的图谱分析表明，先前描述的分子量为12600道尔顿和5300道尔顿、含肾上腺脑啡肽的多肽分别构成了这种较大蛋白质的氨基末端和羧基末端序列。它们通过两个碱性氨基酸残基相连，形成了分子量为18200道尔顿蛋白质的完整序列。这种蛋白质是迄今为止从牛肾上腺髓质中分离出的最大的含脑啡肽多肽，并且是其他几种先前已鉴定的含肾上腺脑啡肽的蛋白质和肽的前体。
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本文引用的文献
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2
Opioid peptides and precursors in the adrenal medulla.肾上腺髓质中的阿片肽及其前体。
Adv Biochem Psychopharmacol. 1980;22:167-79.
3
An about 50,000-dalton protein in adrenal medulla: a common precursor of [Met]- and [Leu]enkephalin.肾上腺髓质中一种约50,000道尔顿的蛋白质：[甲硫氨酸]-脑啡肽和[亮氨酸]-脑啡肽的共同前体。
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4
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Biochem Biophys Res Commun. 1981 Nov 30;103(2):698-705. doi: 10.1016/0006-291x(81)90506-4.
5
Adrenal opioid proteins of 8600 and 12,600 daltons: intermediates in proenkephalin processing.8600道尔顿和12,600道尔顿的肾上腺阿片样蛋白：脑啡肽原加工过程中的中间体。
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6
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