用[35S]甲硫氨酸进行的研究表明，嗜铬细胞中含22,000道尔顿[甲硫氨酸]脑啡肽的蛋白质是[甲硫氨酸]脑啡肽的前体。
Studies with [35S]methionine indicate that the 22,000-dalton [Met]enkephalin-containing protein in chromaffin cells is a precursor of [Met]enkephalin.
作者信息
Rossier J, Trifaró J M, Lewis R V, Lee R W, Stern A, Kimura S, Stein S, Udenfriend S
出版信息
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6889-91. doi: 10.1073/pnas.77.11.6889.
Abstract
It has been shown that [35S]methionine is incorporated into the [Met]enkephalin sequences of a 22,000-dalton enkephalin-containing protein in the adrenal medulla. Pulse-chase experiments indicate that label is incorporated into the large polypeptide before it appears in free [Met]enkephalin and a smaller [Met]enkephalin-containing peptide. These findings provide direct evidence of a precursor-product relationship of these structurally related polypeptides.
摘要
已经表明，[35S]甲硫氨酸被掺入肾上腺髓质中一种22,000道尔顿含脑啡肽蛋白的[甲硫氨酸]脑啡肽序列中。脉冲追踪实验表明，标记物在出现在游离的[甲硫氨酸]脑啡肽和一种较小的含[甲硫氨酸]脑啡肽的肽之前，先被掺入到大的多肽中。这些发现为这些结构相关多肽的前体-产物关系提供了直接证据。
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本文引用的文献
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An about 50,000-dalton protein in adrenal medulla: a common precursor of [Met]- and [Leu]enkephalin.肾上腺髓质中一种约50,000道尔顿的蛋白质：[甲硫氨酸]-脑啡肽和[亮氨酸]-脑啡肽的共同前体。
Science. 1980 Jun 27;208(4451):1459-61. doi: 10.1126/science.7384787.
2
Synthesis of enkephalins by adrenal medullary chromaffin cells: reserpine increases incorporation of radiolabeled amino acids.肾上腺髓质嗜铬细胞中脑啡肽的合成：利血平增加放射性标记氨基酸的掺入。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4364-8. doi: 10.1073/pnas.77.7.4364.
3
Probable precursors of [Leu]enkephalin and [Met]enkephalin in adrenal medulla: peptides of 3-5 kilodaltons.肾上腺髓质中[亮氨酸]脑啡肽和[甲硫氨酸]脑啡肽可能的前体：3至5千道尔顿的肽类。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1681-5. doi: 10.1073/pnas.77.3.1681.
4
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Biochem Biophys Res Commun. 1979 Aug 13;89(3):822-9. doi: 10.1016/0006-291x(79)91852-7.
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