一种用于检测细胞培养物中产生的免疫球蛋白的简单快速乳胶固定试验。
A simple and rapid latex fixation test for measuring immunoglobulins produced in cell cultures.
作者信息
Kasahara T, Harada H, Enomoto H, Itoh Y, Kawai T, Shioiri-Nakano K
出版信息
J Immunol Methods. 1981;47(1):113-20. doi: 10.1016/0022-1759(81)90262-3.
A rapid and simple latex fixation test (LFT), which quantifies immunoglobulin (Ig) released into culture supernatants is described. Latex particles are coated with rabbit anti-human IgG, IgA or IgM antibodies. With this LFT technique the concentration of Ig is determined within a few minutes. The LFT is as sensitive and quantitative as double-antibody radioimmunoassay and is capable of detecting 35, 68 and 225 ng/ml of IgG, IgA and IgM, respectively.
本文描述了一种快速简便的乳胶凝集试验（LFT），该试验可对释放到培养上清液中的免疫球蛋白（Ig）进行定量。乳胶颗粒包被有兔抗人IgG、IgA或IgM抗体。采用这种LFT技术，可在几分钟内测定Ig的浓度。LFT与双抗体放射免疫测定法一样灵敏且具有定量性，分别能够检测到35、68和225 ng/ml的IgG、IgA和IgM。
Zotero 插件