Barta O, Waltman C, Shaffer L M, Oyekan P P
Vet Immunol Immunopathol. 1982 Nov;3(6):567-83. doi: 10.1016/0165-2427(82)90040-x.
The immunoregulatory effect of serum on phytomitogen-induced lymphocyte blastogenesis was studied in 4 sera from diseased dogs and 1 serum from a clinically healthy dog. The results indicated that: (1) Each of the diseased animals responded to the given infection with a specific pattern of blastogenesis inhibition. (2) The blastogenesis suppression in vitro was proportional to the content of the suppressive serum in the medium. (3) A simultaneous presence of the mitogen and the suppressing "serum's lymphocyte immunoregulatory factors" (SLIF) was necessary for inducing blastogenesis suppression. (4) The suppressive sera most probably acted directly on the cells. (5) The final effect of the sera on lymphocyte blastogenesis was a result of an orchestrated action of blastogenesis-supporting, augmenting, and suppressing SLIF cooperating with the mitogen. (6) The suppressive pattern varied with the individual peripheral blood lymphocytes populations used in the test. (7) The blastogenesis-suppressing SLIF was heat-stable, noncytotoxic, and was not or only partially removable by absorption with peripheral blood lymphocytes. (8) The testing of SLIF activities required the use of various animal lymphocytes and a relatively complex setup of mitogens and control serum combinations for correct interpretations.
研究了患病犬的4份血清和1份临床健康犬的血清对植物有丝分裂原诱导的淋巴细胞增殖的免疫调节作用。结果表明：(1) 每只患病动物对特定感染的反应都表现出特定模式的增殖抑制。(2) 体外增殖抑制与培养基中抑制性血清的含量成正比。(3) 有丝分裂原和抑制性 “血清淋巴细胞免疫调节因子” (SLIF) 同时存在是诱导增殖抑制所必需的。(4) 抑制性血清很可能直接作用于细胞。(5) 血清对淋巴细胞增殖的最终作用是支持、增强和抑制SLIF与有丝分裂原协同作用的结果。(6) 抑制模式因试验中使用的个体外周血淋巴细胞群体而异。(7) 抑制增殖的SLIF是热稳定的，无细胞毒性，并且不能或仅部分可通过外周血淋巴细胞吸收去除。(8) 检测SLIF活性需要使用各种动物淋巴细胞以及相对复杂的有丝分裂原和对照血清组合设置才能进行正确解读。
