Guilley H, Briand J P
Cell. 1978 Sep;15(1):113-22. doi: 10.1016/0092-8674(78)90087-9.
The primary structure of the coat protein messenger RNA of turnip yellow mosaic virus is presented. This sequence is the first complete nucleotide sequence of the coat protein messenger of a plant virus to be reported. The coding region, consisting of 567 nucleotides, is flanked by a 5' noncoding region of 19 nucleotides (not including the initiation codon and the cap structure) and by a 3' noncoding region of 109 nucleotides (including the termination signal). The coat protein mRNA has a base composition identical to that of the genome RNA with, in particular, the same high content in cytosine (38%). The codons that govern the incorporation of amino acids into the coat protein are nonrandomly utilized: is greater than 50% of the time the third base of the codons used is a cytosine. This pattern of codon preference is particularly marked for Leu, lle Val, Thr and Cys.
本文给出了芜菁黄花叶病毒外壳蛋白信使核糖核酸的一级结构。该序列是所报道的植物病毒外壳蛋白信使核糖核酸的首个完整核苷酸序列。编码区由567个核苷酸组成，两侧分别是19个核苷酸的5'非编码区（不包括起始密码子和帽结构）以及109个核苷酸的3'非编码区（包括终止信号）。外壳蛋白信使核糖核酸的碱基组成与基因组核糖核酸相同，尤其是胞嘧啶含量同样很高（38%）。决定氨基酸掺入外壳蛋白的密码子并非随机使用：所使用密码子的第三个碱基在超过50%的情况下为胞嘧啶。这种密码子偏好模式在亮氨酸、异亮氨酸、缬氨酸、苏氨酸和半胱氨酸中尤为明显。
