胰岛中5-羟色胺转运的特征
Characteristics of 5-hydroxytryptamine transport in pancreatic islets.
作者信息
Lindström P, Sehlin J, Täljedal I B
出版信息
Br J Pharmacol. 1980 Apr;68(4):773-8. doi: 10.1111/j.1476-5381.1980.tb10871.x.
Abstract
1 Transmembrane transport of 3H-labelled 5-hydroxytryptamine (5-HT) by isolated pancreatic islets of non-inbred ob/ob mice was studied. 2 5-HT was vigorously accumulated in a temperature-dependent way by the islet cells. 3 Studies of the concentration-dependence of [3H]-5-HT uptake revealed complex kinetics with one component being saturated at 1 to 3 microM 5-HT (apparent association constant 0.6 x 10(6) M(-1) and the other non-saturated up to 1 mM 5-HT. 4 The saturable uptake was inhibited by Na+-deficiency and metabolic poisoning with 2,4-dinitrophenol and antimycin A, whereas the non-saturable component was not affected. 5 Omission of K+, Ca2+ or Mg2+ did not affect the uptake rate. 6 It is concluded that 5-HT is taken up by pancreatic beta-cells by mechanisms very similar to those observed in thrombocytes and neurones.
摘要
- 研究了非近交系ob/ob小鼠分离的胰岛对3H标记的5-羟色胺（5-HT）的跨膜转运。2. 胰岛细胞以温度依赖的方式强烈积累5-HT。3. 对[3H]-5-HT摄取的浓度依赖性研究揭示了复杂的动力学，其中一个组分在1至3 microM 5-HT时饱和（表观缔合常数0.6×10(6) M(-1)），另一个组分在高达1 mM 5-HT时不饱和。4. 可饱和摄取受到缺钠以及用2,4-二硝基苯酚和抗霉素A进行代谢中毒的抑制，而非饱和组分不受影响。5. 省略K+、Ca2+或Mg2+不影响摄取速率。6. 得出结论，5-HT被胰腺β细胞摄取的机制与在血小板和神经元中观察到的机制非常相似。
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