利用单克隆抗体快速、可靠地诊断衣原体性眼病。
Rapid, reliable diagnosis of chlamydial ophthalmia by means of monoclonal antibodies.
作者信息
Hawkins D A, Wilson R S, Thomas B J, Evans R T
出版信息
Br J Ophthalmol. 1985 Sep;69(9):640-4. doi: 10.1136/bjo.69.9.640.
Abstract
The use of fluorescein-conjugated monoclonal antibody (Syva, UK) provided a rapid reliable diagnostic test for Chlamydia trachomatis in conjunctival samples from 100 adults with acute follicular conjunctivitis and seven babies with suspected chlamydial ophthalmia neonatorum. Elementary bodies (EBs) were seen in smears from 11 of the adults, and culture confirmed C. trachomatis infection in nine of them. Both tests were positive with specimens from four of the neonates. No specimens from either group of patients produced a negative result in the smear test but a positive result by culture. However, the two adult patients with chlamydial ophthalmia who had negative cultures but were EB-positive had both had prior topical tetracycline therapy.
摘要
使用荧光素结合单克隆抗体（Syva，英国）对100例患有急性滤泡性结膜炎的成人以及7例疑似衣原体性新生儿眼炎的婴儿的结膜样本进行沙眼衣原体检测，提供了一种快速可靠的诊断方法。在11例成人的涂片样本中发现了原体（EBs），其中9例经培养确诊为沙眼衣原体感染。两组新生儿的4份样本两种检测均呈阳性。两组患者的样本在涂片检测中均未出现阴性结果但培养结果为阳性的情况。然而，两名患有衣原体性眼炎的成年患者培养结果为阴性但EB检测呈阳性，他们之前均接受过局部四环素治疗。
相似文献
1
Rapid, reliable diagnosis of chlamydial ophthalmia by means of monoclonal antibodies.利用单克隆抗体快速、可靠地诊断衣原体性眼病。
Br J Ophthalmol. 1985 Sep;69(9):640-4. doi: 10.1136/bjo.69.9.640.
2
3
Diagnosis of ophthalmia neonatorum.新生儿眼炎的诊断。
Br Med J (Clin Res Ed). 1987 Nov 28;295(6610):1377-9. doi: 10.1136/bmj.295.6610.1377.
4
An application of fluorescein-conjugated monoclonal antibodies to the diagnosis of Chlamydia trachomatis infection in children.荧光素偶联单克隆抗体在儿童沙眼衣原体感染诊断中的应用。
Tohoku J Exp Med. 1985 Jan;145(1):57-63. doi: 10.1620/tjem.145.57.
5
Ophthalmia neonatorum associated with maternal chlamydial infections.与母体衣原体感染相关的新生儿眼炎
Trans Sect Ophthalmol Am Acad Ophthalmol Otolaryngol. 1977 Mar-Apr;83(2):302-8.
6
Diagnosis of neonatal chlamydial conjunctivitis.新生儿衣原体性结膜炎的诊断
Arch Dis Child. 1990 Aug;65(8):894-5. doi: 10.1136/adc.65.8.894.
7
Ophthalmia neonatorum due to Chlamydia trachomatis: a family problem?沙眼衣原体所致新生儿眼炎：一个家庭问题？
J Fam Pract. 1979 Jan;8(1):39-43.
8
Ophthalmia neonatorum in Bangkok: the significance of Chlamydia trachomatis.曼谷新生儿眼炎：沙眼衣原体的重要性
Ann Trop Paediatr. 1993;13(3):233-6. doi: 10.1080/02724936.1993.11747651.
9
Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture.采用聚合酶链反应和 McCoy 细胞培养对新生儿衣原体性结膜炎进行比较诊断。
Am J Ophthalmol. 1994 Jan 15;117(1):50-7. doi: 10.1016/s0002-9394(14)73014-4.
10
The detection of Chlamydia trachomatis by direct immunofluorescence in conjunctival smears from patients with trachoma and patients with ophthalmia neonatorum using a conjugated monoclonal antibody.使用结合单克隆抗体，通过直接免疫荧光法检测沙眼患者和新生儿眼炎患者结膜涂片样本中的沙眼衣原体。
J Hyg (Lond). 1986 Feb;96(1):83-7. doi: 10.1017/s0022172400062562.
引用本文的文献
1
Evaluation of patients with dry eye disease for conjunctival and .对干眼症患者进行结膜评估以及……（原文此处不完整）
Int J Ophthalmol. 2016 Oct 18;9(10):1457-1465. doi: 10.18240/ijo.2016.10.15. eCollection 2016.
2
Diagnosis of viral and chlamydial keratoconjunctivitis: which laboratory test?病毒性和衣原体性角结膜炎的诊断：应进行哪种实验室检查？
Br J Ophthalmol. 1999 May;83(5):622-7. doi: 10.1136/bjo.83.5.622.
3
Evaluation of enzyme immunoassay (Chlamydiazyme) for detecting Chlamydia trachomatis in genital tract specimens.评估酶免疫测定法（衣原体酶免疫测定）用于检测生殖道标本中的沙眼衣原体。
J Clin Pathol. 1987 Feb;40(2):194-9. doi: 10.1136/jcp.40.2.194.
4
Evidence that Chlamydia trachomatis causes seronegative arthritis in women.沙眼衣原体导致女性血清阴性关节炎的证据。
Ann Rheum Dis. 1988 Apr;47(4):295-9. doi: 10.1136/ard.47.4.295.
5
Incidence and clinical presentation of chlamydial keratoconjunctivitis: a preliminary study.
Int Ophthalmol. 1988;12(4):201-5. doi: 10.1007/BF00133933.
6
Laboratory techniques for the diagnosis of chlamydial infections.用于诊断衣原体感染的实验室技术。
Genitourin Med. 1991 Jun;67(3):256-66. doi: 10.1136/sti.67.3.256.
本文引用的文献
1
Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization.抗沙眼衣原体单克隆抗体：抗体特异性及抗原特性
J Immunol. 1982 Mar;128(3):1083-9.
2
Comparative sensitivity of fluorescent antibody staining of conjunctival scrapings and irradiated McCoy cell culture for the diagnosis of hyperendemic trachoma.结膜刮片荧光抗体染色与经辐照的 McCoy 细胞培养用于诊断高度流行型沙眼的比较敏感性
Br J Ophthalmol. 1980 Apr;64(4):276-8. doi: 10.1136/bjo.64.4.276.
3
Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies.使用单克隆抗体对沙眼衣原体进行非培养诊断。
N Engl J Med. 1984 May 3;310(18):1146-50. doi: 10.1056/NEJM198405033101803.
4
Adult chlamydial ophthalmia (paratrachoma).
Br Med Bull. 1983 Apr;39(2):123-7. doi: 10.1093/oxfordjournals.bmb.a071802.
5
Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation.使用荧光素标记单克隆抗体检测涂片沙眼衣原体原体的敏感性：与传统衣原体分离法的比较
J Clin Pathol. 1984 Jul;37(7):812-6. doi: 10.1136/jcp.37.7.812.
6
Prevalence of acute conjunctivitis caused by chlamydia, adenovirus, and herpes simplex virus in an ophthalmic casualty department.眼科急诊室中由衣原体、腺病毒和单纯疱疹病毒引起的急性结膜炎患病率。
Br J Ophthalmol. 1984 Sep;68(9):653-5. doi: 10.1136/bjo.68.9.653.
7
Human serology in Chlamydia trachomatis infection with microimmunofluorescence.沙眼衣原体感染的微量免疫荧光法人体血清学研究
J Infect Dis. 1974 Oct;130(4):388-97. doi: 10.1093/infdis/130.4.388.
8
Evaluation of laboratory methods for detecting acute TRIC agent infection.检测急性TRIC病原体感染的实验室方法评估
Am J Ophthalmol. 1970 Sep;70(3):375-80. doi: 10.1016/0002-9394(70)90097-8.
9
Rapid serological test for diagnosis of chlamydial ocular infections.用于诊断衣原体眼部感染的快速血清学检测
Br J Ophthalmol. 1978 Aug;62(8):503-8. doi: 10.1136/bjo.62.8.503.
10
Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining.结合使用环己酰亚胺处理的 McCoy 细胞和免疫荧光染色法对衣原体包涵体进行早期检测。
J Clin Microbiol. 1977 Sep;6(3):285-92. doi: 10.1128/jcm.6.3.285-292.1977.
Zotero 插件