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从接种的冷却塔水中分离嗜肺军团菌的阴性富集程序。

Negative enrichment procedure for isolation of Legionella pneumophila from seeded cooling tower water.

作者信息

Thorpe T C, Miller R D

出版信息

Appl Environ Microbiol. 1980 Oct;40(4):849-51. doi: 10.1128/aem.40.4.849-851.1980.

DOI:10.1128/aem.40.4.849-851.1980
PMID:7000000
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC291670/
Abstract

A negative enrichment procedure was developed which was capable of isolating Legionella pneumophila directly from seeded air-conditioning cooling tower water onto laboratory media. This procedure was based on an 8-h incubation under conditions that were bactericidal to the indigenous water microflora but merely bacteriostatic to L. pneumophila.

摘要

开发了一种阴性富集程序,该程序能够将嗜肺军团菌直接从接种的空调冷却塔水中分离到实验室培养基上。该程序基于在对本地水微生物群落具有杀菌作用但对嗜肺军团菌仅具有抑菌作用的条件下进行8小时培养。

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Negative enrichment procedure for isolation of Legionella pneumophila from seeded cooling tower water.从接种的冷却塔水中分离嗜肺军团菌的阴性富集程序。
Appl Environ Microbiol. 1980 Oct;40(4):849-51. doi: 10.1128/aem.40.4.849-851.1980.
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Appl Environ Microbiol. 1983 Mar;45(3):1119-21. doi: 10.1128/aem.45.3.1119-1121.1983.
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引用本文的文献

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J Clin Microbiol. 1981 Sep;14(3):298-303. doi: 10.1128/jcm.14.3.298-303.1981.
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Antiserum-agar plate method for simultaneous detection and direct isolation of Legionella species in clinical and environmental specimens.抗血清琼脂平板法用于临床和环境标本中军团菌属的同步检测和直接分离。
J Clin Microbiol. 1982 Jun;15(6):1176-7. doi: 10.1128/jcm.15.6.1176-1177.1982.
3
A prevalence study of antibodies to Legionella spp. in geriatric institutions.老年机构中军团菌属抗体的患病率研究。
J Hyg (Lond). 1984 Feb;92(1):37-43. doi: 10.1017/s0022172400064007.
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Comparison of culture methods and an immunofluorescence assay for the detection of Legionella pneumophila in domestic hot water devices.用于检测家用热水设备中嗜肺军团菌的培养方法与免疫荧光检测法的比较
Curr Microbiol. 1992 Jul;25(1):19-23. doi: 10.1007/BF01570077.

本文引用的文献

1
An outbreak of Legionnaires' disease associated with a contaminated air-conditioning cooling tower.一起与受污染的空调冷却塔相关的军团病暴发。
N Engl J Med. 1980 Feb 14;302(7):365-70. doi: 10.1056/NEJM198002143020703.
2
Use of a semiselective medium to culture Legionella pneumophila from contaminated lung specimens.使用半选择性培养基从受污染的肺标本中培养嗜肺军团菌。
J Clin Microbiol. 1979 Aug;10(2):141-3. doi: 10.1128/jcm.10.2.141-143.1979.
3
Growth of Legionnaires disease bacterium (Legionella pneumophila) in chemically defined medium.嗜肺军团菌在化学成分确定的培养基中的生长情况。
J Clin Microbiol. 1979 Jul;10(1):50-5. doi: 10.1128/jcm.10.1.50-55.1979.
4
Epidemiology of Legionnaires' disease.军团病流行病学
Ann Intern Med. 1979 Apr;90(4):499-502. doi: 10.7326/0003-4819-90-4-499.
5
Isolation of Legionella pneumophila from nonepidemic-related aquatic habitats.从与疫情无关的水生栖息地中分离嗜肺军团菌。
Appl Environ Microbiol. 1979 Jun;37(6):1239-42. doi: 10.1128/aem.37.6.1239-1242.1979.