在使用豚鼠从冷却塔水中分离嗜肺军团菌方面，设施和灵敏度得到了改进。
Improved facility and sensitivity in the use of guinea pigs for the isolation of Legionella pneumophila from cooling tower water.
作者信息
Leinbach E D, Winkler H H, Wood D O, Coggin J H
出版信息
Appl Environ Microbiol. 1983 Mar;45(3):1119-21. doi: 10.1128/aem.45.3.1119-1121.1983.
Abstract
The established criteria for the determination of the optimum time for the sacrifice of guinea pigs inoculated with samples of cooling tower water were found to be inadequate for the detection of low levels of Legionella pneumophila. By ignoring the requirement for fever and by sequentially sacrificing the infected guinea pigs on days 3 through 5 postinoculation, we simplified the procedure, and the sensitivity of detection was improved a great deal.
摘要
已确立的用于确定接种冷却塔水样的豚鼠最佳处死时间的标准，被发现不足以检测低水平的嗜肺军团菌。通过忽略发热要求，并在接种后第3天至第5天依次处死受感染的豚鼠，我们简化了程序，检测灵敏度得到了极大提高。
相似文献
1
Improved facility and sensitivity in the use of guinea pigs for the isolation of Legionella pneumophila from cooling tower water.
Appl Environ Microbiol. 1983 Mar;45(3):1119-21. doi: 10.1128/aem.45.3.1119-1121.1983.
2
Isolation of Legionella pneumophilia from cooling tower water by filtration.
Appl Environ Microbiol. 1981 May;41(5):1202-5. doi: 10.1128/aem.41.5.1202-1205.1981.
3
Negative enrichment procedure for isolation of Legionella pneumophila from seeded cooling tower water.
Appl Environ Microbiol. 1980 Oct;40(4):849-51. doi: 10.1128/aem.40.4.849-851.1980.
4
[Isolation of Legionella pneumophila from cooling tower (author's transl)].
Kansenshogaku Zasshi. 1981 Jun;55(6):439-46. doi: 10.11150/kansenshogakuzasshi1970.55.439.
5
[Isolation of Legionella pneumophila from the UOEH cooling tower].
J UOEH. 1986 Dec 1;8(4):423-8. doi: 10.7888/juoeh.8.423.
6
[Isolation of Legionella from the water in the cooling tower of an air-conditioning system].
Zhonghua Liu Xing Bing Xue Za Zhi. 1985 Aug;6(4):193-5.
7
Isolation of Legionella pneumophila from water supplies: comparison of methods based on the guinea-pig and culture media.
J Hyg (Lond). 1983 Oct;91(2):179-87. doi: 10.1017/s0022172400060186.
8
Legionella spp. in Puerto Rico cooling towers.
Appl Environ Microbiol. 1988 Oct;54(10):2331-4. doi: 10.1128/aem.54.10.2331-2334.1988.
9
[Isolation of Legionella pneumophila from cooling tower in China].
Kansenshogaku Zasshi. 1985 Aug;59(8):787-92. doi: 10.11150/kansenshogakuzasshi1970.59.787.
10
Isolation of Legionella pneumophila from hospital potable water specimens: comparison of direct plating with guinea pig inoculation.
J Clin Microbiol. 1982 Jun;15(6):1092-6. doi: 10.1128/jcm.15.6.1092-1096.1982.
引用本文的文献
1
Intracellular growth of Legionella pneumophila within Acanthamoeba castellanii Neff.
Infect Immun. 1984 Jul;45(1):18-24. doi: 10.1128/iai.45.1.18-24.1984.
2
Comparison of culture methods and an immunofluorescence assay for the detection of Legionella pneumophila in domestic hot water devices.
Curr Microbiol. 1992 Jul;25(1):19-23. doi: 10.1007/BF01570077.
本文引用的文献
1
Glycine-containing selective medium for isolation of Legionellaceae from environmental specimens.
Appl Environ Microbiol. 1981 Nov;42(5):768-72. doi: 10.1128/aem.42.5.768-772.1981.
2
Pontiac fever: isolation of the etiologic agent (Legionella pneumophilia) and demonstration of its mode of transmission.
Am J Epidemiol. 1981 Sep;114(3):337-47. doi: 10.1093/oxfordjournals.aje.a113200.
3
Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens.
J Clin Microbiol. 1981 Sep;14(3):298-303. doi: 10.1128/jcm.14.3.298-303.1981.
4
Isolation of Legionella spp. from environmental water samples by low-pH treatment and use of a selective medium.
J Clin Microbiol. 1981 Apr;13(4):714-9. doi: 10.1128/jcm.13.4.714-719.1981.
5
Virulence conversion of Legionella pneumophila serogroup 1 by passage in guinea pigs and embryonated eggs.
Infect Immun. 1982 Mar;35(3):943-6. doi: 10.1128/iai.35.3.943-946.1982.
6
Ecological distribution of Legionella pneumophila.
Appl Environ Microbiol. 1981 Jan;41(1):9-16. doi: 10.1128/aem.41.1.9-16.1981.
7
Legionella pneumophila in water plumbing systems.
Lancet. 1982 Apr 24;1(8278):949-51. doi: 10.1016/s0140-6736(82)91944-4.
8
Isolation of the Legionnaires' disease bacterium from environmental samples.
Ann Intern Med. 1979 Apr;90(4):664-6. doi: 10.7326/0003-4819-90-4-664.
Zotero 插件