Vos J G
Arch Toxicol Suppl. 1980;4:95-108. doi: 10.1007/978-3-642-67729-8_25.
As a screening procedure to detect immunotoxic effects, different parameters are discussed. These criteria include growth, weight and histology of lymphoid and endocrine organs, haematology, as well s serum immunoglobulin concentrations which can be quantified using the enzyme-linked immunosorbent assay (ELISA). Secondly, different function tests are discussed to assess in the rat the cell-mediated immunity, the humoral immunity and the phagocytosis by macrophages. These tests should be performed when in a screening study an indication of immunotoxicity is found at a relevant dose level. The aim of functional assessment is to determine the functional significance of an effect on the immune response and to gain more insight into the mode of action of the chemical. Finally, examples are given of compounds that suppress or stimulate immune responses; and advantages are discussed of performing function tests during the development phase of the immune system.
作为检测免疫毒性效应的筛查程序，人们讨论了不同的参数。这些标准包括淋巴和内分泌器官的生长、重量及组织学、血液学，以及可使用酶联免疫吸附测定（ELISA）进行定量的血清免疫球蛋白浓度。其次，讨论了不同的功能测试，以评估大鼠的细胞介导免疫、体液免疫和巨噬细胞的吞噬作用。当在筛查研究中在相关剂量水平发现免疫毒性迹象时，应进行这些测试。功能评估的目的是确定对免疫反应影响的功能意义，并更深入地了解该化学物质的作用方式。最后，给出了抑制或刺激免疫反应的化合物实例，并讨论了在免疫系统发育阶段进行功能测试的优点。
